Abstract
The down-regulation of dominant oncogenes, including C-MYC, in tumor cells often leads to the induction of senescence via mechanisms that are not completely identified. In the current study, we demonstrate that MYC-depleted melanoma cells undergo extensive DNA damage that is caused by the underexpression of thymidylate synthase (TS) and ribonucleotide reductase (RR) and subsequent depletion of deoxyribonucleoside triphosphate pools. Simultaneous genetic inhibition of TS and RR in melanoma cells induced DNA damage and senescence phenotypes very similar to the ones caused by MYC-depletion. Reciprocally, overexpression of TS and RR in melanoma cells or addition of deoxyribo-nucleosides to culture media substantially inhibited DNA damage and senescence-associated phenotypes caused by C-MYC depletion. Our data demonstrate the essential role of TS and RR in C-MYC-dependent suppression of senescence in melanoma cells.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Cell Line, Tumor
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Cellular Senescence / drug effects*
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DNA Damage / drug effects*
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Deoxyribonucleosides / pharmacology*
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Down-Regulation
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Gene Expression Regulation, Neoplastic
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Genotype
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Humans
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Melanoma / enzymology*
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Melanoma / genetics
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Melanoma / pathology
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Phenotype
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Proto-Oncogene Proteins c-myc / genetics
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Proto-Oncogene Proteins c-myc / metabolism*
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RNA Interference
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Ribonucleoside Diphosphate Reductase / metabolism
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Ribonucleotide Reductases / genetics
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Ribonucleotide Reductases / metabolism*
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Skin Neoplasms / enzymology*
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Skin Neoplasms / genetics
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Skin Neoplasms / pathology
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Thymidylate Synthase / genetics
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Thymidylate Synthase / metabolism*
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Time Factors
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Transfection
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Tumor Suppressor Proteins / metabolism
Substances
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Deoxyribonucleosides
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MYC protein, human
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Proto-Oncogene Proteins c-myc
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Tumor Suppressor Proteins
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Ribonucleotide Reductases
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ribonucleotide reductase M2
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RRM1 protein, human
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Ribonucleoside Diphosphate Reductase
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Thymidylate Synthase