Abstract
Alpha thalassemia/mental retardation syndrome X-linked (ATRX) is a member of the SWI/SNF protein family of DNA-dependent ATPases. It functions as a chromatin remodeler and is classified as an SNF2-like helicase. Here, we showed somatic knock-out of ATRX displayed perturbed S-phase progression as well as hypersensitivity to replication stress. ATRX is recruited to sites of DNA damage, required for efficient checkpoint activation and faithful replication restart. In addition, we identified ATRX as a binding partner of MRE11-RAD50-NBS1 (MRN) complex. Together, these results suggest a non-canonical function of ATRX in guarding genomic stability.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Acid Anhydride Hydrolases
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism
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DNA Damage
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DNA Helicases / genetics
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DNA Helicases / metabolism*
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DNA Repair Enzymes / genetics
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DNA Repair Enzymes / metabolism
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DNA Replication / physiology*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Gene Knockdown Techniques
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Genomic Instability / physiology*
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HeLa Cells
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Humans
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MRE11 Homologue Protein
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Multiprotein Complexes / genetics
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Multiprotein Complexes / metabolism
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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S Phase / physiology*
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X-linked Nuclear Protein
Substances
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Cell Cycle Proteins
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DNA-Binding Proteins
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MRE11 protein, human
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Multiprotein Complexes
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NBN protein, human
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Nuclear Proteins
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MRE11 Homologue Protein
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Acid Anhydride Hydrolases
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RAD50 protein, human
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DNA Helicases
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ATRX protein, human
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X-linked Nuclear Protein
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DNA Repair Enzymes