Abstract
Human gene icb-1 has been originally identified to be involved in differentiation processes of cancer cells. To examine the function of icb-1 in ovarian cancer, we knocked down its expression in three ovarian cancer cell lines and performed microarray-based gene expression profiling with subsequent gene network modeling. Loss of icb-1 expression accelerated proliferation of SK-OV-3, OVCAR-3 and OAW-42 cells and led to upregulation of ovarian cancer biomarkers like KLK10 and CLDN16. Most of the upregulated genes were part of oncogenic pathways regulated by ERα or TNF. Our data suggest that icb-1 gene inhibits growth and progression of ovarian cancer cells.
Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
MeSH terms
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Cell Differentiation / genetics
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Cell Line, Tumor
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Cell Proliferation*
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Claudins / biosynthesis
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Estrogen Receptor alpha / metabolism
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Female
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Gene Expression Profiling
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Gene Expression Regulation, Neoplastic
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Humans
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Intracellular Signaling Peptides and Proteins / genetics
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Intracellular Signaling Peptides and Proteins / metabolism*
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Kallikreins / biosynthesis
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Neoplasm Proteins / genetics
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Neoplasm Proteins / metabolism*
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Ovarian Neoplasms / genetics*
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RNA Interference
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RNA, Small Interfering
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Tumor Necrosis Factor-alpha / metabolism
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Up-Regulation
Substances
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Claudins
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ESR1 protein, human
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Estrogen Receptor alpha
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Intracellular Signaling Peptides and Proteins
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Neoplasm Proteins
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RNA, Small Interfering
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THEMIS2 protein, human
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Tumor Necrosis Factor-alpha
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claudin 16
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KLK10 protein, human
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Kallikreins