Abstract
We have isolated and characterized a human ROS1 cDNA from the glioblastoma cell line SW-1088. The cDNA, 8.3 kilobases long, has the potential to encode a transmembrane tyrosine-specific protein kinase with a predicted molecular mass of 259 kDa. The putative extracellular domain of ROS1 is homologous to the extracellular domain of the sevenless gene product from Drosophila. No comparable similarities in the extracellular domains were found between ROS1 and other receptor-type tyrosine kinases. Together, ROS1 and sevenless gene products define a distinct subclass of transmembrane tyrosine kinases.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cell Line
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DNA, Neoplasm / genetics*
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DNA, Neoplasm / isolation & purification
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Gene Library
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Glioma
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Humans
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Molecular Sequence Data
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Oligonucleotide Probes
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Oncogene Proteins, Viral / genetics*
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Oncogenes*
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Protein Conformation
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Protein-Tyrosine Kinases / genetics
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RNA, Neoplasm / genetics
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RNA, Neoplasm / isolation & purification
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Receptor Protein-Tyrosine Kinases*
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Sequence Homology, Nucleic Acid
Substances
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DNA, Neoplasm
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Oligonucleotide Probes
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Oncogene Proteins, Viral
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RNA, Neoplasm
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Protein-Tyrosine Kinases
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Receptor Protein-Tyrosine Kinases
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v-ros protein, Avian sarcoma virus UR2