Knockdown of ZFX inhibits gastric cancer cell growth in vitro and in vivo via downregulating the ERK-MAPK pathway

Cancer Lett. 2013 Sep 1;337(2):293-300. doi: 10.1016/j.canlet.2013.04.003. Epub 2013 Apr 13.

Abstract

Zinc finger protein X-linked (ZFX) is a zinc finger transcription factor encoded on the X chromosome. Here, we found that ZFX expression was significantly upregulated in gastric cancer (GC) cell lines and tissues. Knockdown of ZFX induced significant apoptosis and cell cycle arrest in SGC7901 and MGC803 cells. Moreover, we demonstrated for the first time that knockdown of ZFX inhibited gastric cancer cell growth in vitro and in vivo via downregulating the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK-MAPK) pathway. Therefore, ZFX play a prominent role in GC tumorigenicity and may have potential applications in the diagnosis or treatment of GC.

Keywords: ERK-MAPK; Gastric cancer (GC); Zinc finger protein X-linked (ZFX).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis
  • Cell Cycle Checkpoints
  • Cell Line, Tumor
  • Cell Proliferation*
  • Down-Regulation
  • Extracellular Signal-Regulated MAP Kinases / metabolism*
  • Female
  • Gene Knockdown Techniques*
  • Genetic Therapy / methods*
  • Humans
  • Kruppel-Like Transcription Factors / genetics
  • Kruppel-Like Transcription Factors / metabolism*
  • MAP Kinase Signaling System*
  • Male
  • Mice
  • Mice, Nude
  • Middle Aged
  • RNA Interference*
  • Stomach Neoplasms / enzymology
  • Stomach Neoplasms / genetics
  • Stomach Neoplasms / pathology
  • Stomach Neoplasms / therapy*
  • Time Factors
  • Transfection
  • Tumor Burden

Substances

  • Kruppel-Like Transcription Factors
  • zinc finger protein, X-linked
  • Extracellular Signal-Regulated MAP Kinases