Abstract
Current influenza vaccines stimulate neutralising antibody to the haemagglutinin antigen but as there is antigenic drift in HA it is difficult to prepare a vaccine in advance against an emergent strain. A potential strategy is to induce CD8(+) and CD4(+) T cells that recognize epitopes within internal proteins that are less subject to antigenic drift. Augmenting humoral responses to HA with T cell responses to more conserved antigens may result in a more broadly protective vaccine. In this study, we evaluate the quality of influenza specific T cell responses in a clinical trial using MVA-NP+M1 vaccination followed by influenza virus challenge. In vaccinated volunteers, the expression of Granzyme A, Perforin and CD57 on influenza HLA A*02 M158-66 antigen specific cells was higher than non-vaccinated volunteers before and after challenge despite a similar frequency of antigen specific cells. BCL2 expression was lower in vaccinated volunteers. These data indicate that antigen specific T cells are a useful additional measure for use in human vaccination or immunization studies.
Publication types
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Clinical Trial
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Antigens, Viral / genetics
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Antigens, Viral / immunology*
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CD57 Antigens / genetics
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CD57 Antigens / immunology
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CD8-Positive T-Lymphocytes / immunology*
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CD8-Positive T-Lymphocytes / virology
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Epitopes, T-Lymphocyte / genetics
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Epitopes, T-Lymphocyte / immunology*
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Granzymes / genetics
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Granzymes / immunology
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HLA-A2 Antigen / genetics
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HLA-A2 Antigen / immunology
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Hemagglutinins / genetics
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Hemagglutinins / immunology
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Humans
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Influenza Vaccines / administration & dosage*
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Influenza Vaccines / genetics
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Influenza Vaccines / immunology
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Influenza, Human / genetics
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Influenza, Human / immunology
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Influenza, Human / prevention & control*
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Middle Aged
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Nucleoproteins / genetics
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Nucleoproteins / immunology
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Orthomyxoviridae / drug effects
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Orthomyxoviridae / immunology
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Perforin / genetics
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Perforin / immunology
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Vaccination*
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Vaccines, DNA
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Vaccines, Synthetic
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Viral Core Proteins / genetics
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Viral Core Proteins / immunology
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Viral Matrix Proteins / genetics
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Viral Matrix Proteins / immunology
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Viral Vaccines / administration & dosage*
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Viral Vaccines / genetics
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Viral Vaccines / immunology
Substances
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Antigens, Viral
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CD57 Antigens
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Epitopes, T-Lymphocyte
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HLA-A2 Antigen
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Hemagglutinins
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Influenza Vaccines
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M1 protein, Influenza A virus
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MVA vaccine
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Nucleoproteins
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Recombinant Proteins
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Vaccines, DNA
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Vaccines, Synthetic
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Viral Core Proteins
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Viral Matrix Proteins
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Viral Vaccines
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Perforin
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Granzymes