Stimulation of phospholipase C-gamma 1 membrane association by epidermal growth factor

Science. 1990 Jul 20;249(4966):296-8. doi: 10.1126/science.2374928.

Abstract

Epidermal growth factor (EGF) treatment of A-431 epidermoid carcinoma cells elicited a redistribution of phospholipase C-gamma 1 (PLC-gamma 1) from a predominantly cytosolic localization to membrane fractions. The temporal coincidence of this redistribution with EGF stimulation of inositol phosphate formation and EGF increased phosphorylation of PLC-gamma 1 suggests that the membrane association of PLC-gamma 1 is a significant event in second messenger transduction.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Carcinoma, Squamous Cell
  • Cell Line
  • Cell Membrane / drug effects
  • Cell Membrane / enzymology
  • Cytosol / enzymology
  • Epidermal Growth Factor / pharmacology*
  • Humans
  • Isoenzymes / metabolism*
  • Kinetics
  • Phosphopeptides / isolation & purification
  • Protein Binding
  • Trypsin
  • Type C Phospholipases / metabolism*

Substances

  • Isoenzymes
  • Phosphopeptides
  • Epidermal Growth Factor
  • Type C Phospholipases
  • Trypsin