Previous studies indicated that the human multidrug resistance 1 gene (MDR1) is an important candidate gene influencing the susceptibility to breast cancer. The purpose of this study was to detect the association between the MDR1 genetic variants and breast cancer susceptibility. A total of 647 subjects consisted of 322 patients with breast cancer and 325 cancer-free healthy controls were enrolled in this study. Data about the risk factors of breast cancer were summarized by questionnaires. The c.1465C>T genetic variant in MDR1 were genotyped by created restriction site-PCR (CRS-PCR) and verified by DNA sequencing methods. No significant differences in the allelic and genotypic frequencies of c.1465C>T polymorphism were detected between breast cancer patients and healthy controls. Our data suggest that there are no significant differences about the distribution of the risk factors of breast cancer patients among CC, CT and TT genotypes. These preliminary results from this study indicate that the c.1465C>T variant is not significantly associated with breast cancer susceptibility in the studied populations.
Keywords: Breast cancer; Cancer susceptibility; MDR1 gene; Risk factors; Single nucleotide polymorphisms.
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