K-sam, an amplified gene in stomach cancer, is a member of the heparin-binding growth factor receptor genes

Proc Natl Acad Sci U S A. 1990 Aug;87(15):5983-7. doi: 10.1073/pnas.87.15.5983.

Abstract

DNA fragments amplified in a stomach cancer-derived cell line, KATO-III, were previously identified by the in-gel DNA renaturation method, and a 0.2-kilobase-pair fragment of the amplified sequence was subsequently cloned. By genomic walking, a portion of the exon of the gene flanking this 0.2-kilobase-pair fragment was cloned, and the gene was designated as K-sam (KATO-III cell-derived stomach cancer amplified gene). The K-sam cDNAs, corresponding to the 3.5-kilobase K-sam mRNA, were cloned from the KATO-III cells. Sequence analysis revealed that this gene coded for 682 amino acid residues that satisfied the characteristics of the receptor tyrosine kinase. The K-sam gene had significant homologies with bek, FLG, and chicken basic fibroblast growth factor receptor gene. The K-sam gene was amplified in KATO-III cells with the major transcript of 3.5-kilobases in size. This gene was also expressed in some other stomach cancer cells, a small cell lung cancer, and germ cell tumors.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Cell Line
  • DNA, Neoplasm / genetics
  • Filaggrin Proteins
  • Gene Amplification*
  • Growth Substances / metabolism
  • Humans
  • Mice
  • Mice, Nude
  • Molecular Sequence Data
  • Multigene Family*
  • Neoplasm Transplantation
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / isolation & purification
  • Receptors, Mitogen / genetics*
  • Receptors, Vascular Endothelial Growth Factor
  • Sequence Homology, Nucleic Acid
  • Stomach Neoplasms / genetics*
  • Transplantation, Heterologous

Substances

  • DNA, Neoplasm
  • FLG protein, human
  • Filaggrin Proteins
  • Growth Substances
  • RNA, Neoplasm
  • Receptors, Mitogen
  • Receptors, Vascular Endothelial Growth Factor

Associated data

  • GENBANK/M35718