Abstract
Clear cell renal cell carcinoma (ccRCC) is the most common histological subtype of kidney cancer and is often characterized by mutations or deletions of the Von Hippel Lindau (VHL) tumour suppressor gene. Aurora gene family members are implicated in proper mitotic progression and spindle checkpoint function and play a crucial role in cancer progression. In the present study, we assessed the expression of Aurora-A in a cohort of 30 ccRCC with fully characterized VHL status (wt/wt or mut/del) and Fuhrman grade. Aurora-A transcript and protein levels were significantly increased in high Fuhrman grade tumours and in VHLwt/wt tumours. These results suggest that Aurora-A and VHL interact in the ccRCC. We demonstrated that the two proteins interact in vivo and identified the Ser72 on the sequence of VHL as the unique site phosphorylated by Aurora-A.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Aurora Kinase A / genetics
-
Aurora Kinase A / metabolism*
-
Carcinoma, Renal Cell / genetics
-
Carcinoma, Renal Cell / metabolism*
-
Carcinoma, Renal Cell / pathology
-
Cell Line, Tumor
-
Gene Expression Regulation, Neoplastic
-
Humans
-
Kidney Neoplasms / genetics
-
Kidney Neoplasms / metabolism*
-
Kidney Neoplasms / pathology
-
Models, Biological
-
Mutation
-
Neoplasm Grading
-
Phosphorylation
-
Protein Binding
-
Transcription, Genetic
-
Von Hippel-Lindau Tumor Suppressor Protein / genetics
-
Von Hippel-Lindau Tumor Suppressor Protein / metabolism*
Substances
-
Von Hippel-Lindau Tumor Suppressor Protein
-
Aurora Kinase A
Grants and funding
This work was supported by the CNRS, the Ligue Nationale Contre le Cancer. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.