Abstract
A novel human Middle East respiratory syndrome coronavirus (MERS-CoV) caused outbreaks of severe acute respiratory syndrome (SARS)-like illness with a high mortality rate, raising concerns of its pandemic potential. Dipeptidyl peptidase-4 (DPP4) was recently identified as its receptor. Here we showed that residues 377 to 662 in the S protein of MERS-CoV specifically bound to DPP4-expressing cells and soluble DPP4 protein and induced significant neutralizing antibody responses, suggesting that this region contains the receptor-binding domain (RBD), which has a potential to be developed as a MERS-CoV vaccine.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Neutralizing / blood
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Antibodies, Viral / blood
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Binding Sites
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Cell Line
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Coronavirus / genetics
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Coronavirus / immunology
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Coronavirus / metabolism*
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Coronavirus Infections / virology
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Dipeptidyl Peptidase 4 / metabolism
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Humans
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Mice
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Mice, Inbred BALB C
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Protein Structure, Tertiary
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Receptors, Virus / metabolism
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Respiratory Tract Infections / virology
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Spike Glycoprotein, Coronavirus / genetics
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Spike Glycoprotein, Coronavirus / immunology
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Spike Glycoprotein, Coronavirus / metabolism*
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Viral Vaccines / genetics
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Viral Vaccines / immunology
Substances
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Antibodies, Neutralizing
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Antibodies, Viral
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Receptors, Virus
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Spike Glycoprotein, Coronavirus
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Viral Vaccines
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DPP4 protein, human
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Dipeptidyl Peptidase 4