Molecular cloning and sequence analysis of cDNA encoding human cholesterol 7 alpha-hydroxylase

FEBS Lett. 1990 Jul 30;268(1):137-40. doi: 10.1016/0014-5793(90)80992-r.

Abstract

A complete cDNA clone encoding human cholesterol 7 alpha-hydroxylase has been isolated using a rat P-450ch7 alpha cDNA insert [(1989) FEBS Lett. 257, 97-100] as a probe and totally sequenced. The cDNA contained 1512-base pair open reading frame encoding 504 amino acid residues (Mr 57,630), 39-base pair 5'-untranslated region 1322-base pair 3'-ultranslated region including 20 nucleotides of poly A tail in the total length of 2873 base pairs. The deduced amino acid sequence showed 82% similarity to rat P-450ch7 alpha. Unique amino acid residues were observed in putative binding domains for heme and steroid which are highly conserved in most steroidogenic P-450s.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Cells, Cultured
  • Cholesterol 7-alpha-Hydroxylase / genetics*
  • Cloning, Molecular
  • DNA / genetics
  • Gene Expression
  • Humans
  • Liver / enzymology
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • Steroid Hydroxylases / genetics*

Substances

  • RNA, Messenger
  • DNA
  • Steroid Hydroxylases
  • Cholesterol 7-alpha-Hydroxylase

Associated data

  • GENBANK/X56088