Abstract
We have previously reported that lck mRNA (a lymphocyte-specific protein tyrosine kinase gene) is absent in human T-cell leukemia virus type I (HTLV-I)-infected interleukin-2(IL-2)-independent T-cell lines, while HTLV-I-negative T-cell lines and HTLV-I-positive IL-2-dependent ones express a large amount of lck mRNA. To further investigate the levels of lck expression, we prepared rabbit anti-Lck antiserum directed against the synthetic oligopeptide of 32 amino acids corresponding to the carboxy terminus of this gene product, p56lck. Using this antiserum, we show that HTLV-I-positive T-cell lines, whether they are IL-2-dependent or not, scarcely express p56lck. In other words, IL-2-dependent HTLV-I-positive T-cell lines seldom produce p56lck in spite of high expression of lck mRNA. Absence of p56lck is suspected of playing an important role in malignant transformation of HTLV-I-infected T-cells.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Blotting, Northern
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Cell Line
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Cells, Cultured
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Gene Expression Regulation, Viral / genetics*
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Gene Expression Regulation, Viral / immunology
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HTLV-I Infections / genetics*
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HTLV-I Infections / immunology
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Humans
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Immunoblotting
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Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
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Oncogene Proteins, Viral / analysis
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Oncogene Proteins, Viral / genetics*
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Oncogene Proteins, Viral / immunology
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Protein Biosynthesis
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Protein-Tyrosine Kinases / analysis
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Protein-Tyrosine Kinases / genetics*
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Protein-Tyrosine Kinases / immunology
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RNA, Messenger / analysis
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RNA, Messenger / genetics*
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RNA, Messenger / immunology
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RNA, Viral / analysis
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RNA, Viral / genetics*
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RNA, Viral / immunology
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T-Lymphocytes / immunology*
Substances
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Oncogene Proteins, Viral
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RNA, Messenger
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RNA, Viral
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Protein-Tyrosine Kinases
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Lymphocyte Specific Protein Tyrosine Kinase p56(lck)