pstS1 polymorphisms of Mycobacterium tuberculosis strains may reflect ongoing immune evasion

Haican Liu; Yi Jiang; Xiangfeng Dou; Haiyin Wang; Xiuqin Zhao; Wen Zhang; Li Wan; Zhijian Zhang; Chen Chen; Kanglin Wan

doi:10.1016/j.tube.2013.05.003

pstS1 polymorphisms of Mycobacterium tuberculosis strains may reflect ongoing immune evasion

Tuberculosis (Edinb). 2013 Sep;93(5):475-81. doi: 10.1016/j.tube.2013.05.003. Epub 2013 Jul 11.

Authors

Haican Liu¹, Yi Jiang, Xiangfeng Dou, Haiyin Wang, Xiuqin Zhao, Wen Zhang, Li Wan, Zhijian Zhang, Chen Chen, Kanglin Wan

Affiliation

¹ National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, PR China. liuhaican@126.com

PMID: 23849889
DOI: 10.1016/j.tube.2013.05.003

Abstract

The mycobacterial antigen PstS1 is a highly immunogenic and immunostimulatory component of the mycobacterial cell membrane and a good candidate for the diagnosis and vaccination against tuberculosis. Here we selected 180 clinical isolates of Mycobacterium tuberculosis complex (MTBC) in China and 11 different Bacille Calmette Guerin (BCG) strains, amplified the gene of the PstS1 antigen and compared the sequences with those of four other Mycobacterium bovis and BCG strains from the NCBI genome website. Some of the mutations, especially 2 frameshift mutations, occurred in the PstS1antigen, which may have resulted in the protein function alteration and ongoing immune evasion. A unique single nucleotide polymorphism of the M. bovis and BCG strains was found in this antigen and may be useful for differentiating M. bovis and BCG strains from M. tuberculosis strains.

Keywords: Immune evasion; Mycobacterium tuberculosis complex; PstS1 polymorphisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

ATP-Binding Cassette Transporters / genetics*
ATP-Binding Cassette Transporters / metabolism
Amino Acid Sequence
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Bacterial Typing Techniques / methods
Epitopes, T-Lymphocyte / genetics
Humans
Immune Evasion / genetics*
Molecular Sequence Data
Mutation
Mycobacterium bovis / classification
Mycobacterium bovis / genetics
Mycobacterium tuberculosis / classification
Mycobacterium tuberculosis / genetics*
Mycobacterium tuberculosis / immunology
Mycobacterium tuberculosis / metabolism
Polymorphism, Single Nucleotide*
Sequence Alignment

Substances

ATP-Binding Cassette Transporters
Bacterial Proteins
Epitopes, T-Lymphocyte
PstS-1 protein, Mycobacterium tuberculosis