Abstract
P-glycoprotein (Pgp) and XIAP co-expression has been discussed in the process of the acquisition of multidrug resistance (MDR) in cancer. Here, we evaluated XIAP and Pgp expression in chronic myeloid leukemia (CML) samples, showing a positive correlation between them. Furthermore, we evaluated the effects of imatinib in XIAP and Pgp expression using CML cell lines K562 (Pgp(-)) and K562-Lucena (Pgp(+)). Imatinib increased XIAP and Pgp expression in K562-Lucena cells, while in K562 cells a downregulation of these proteins was observed, suggesting that imatinib induces an increment of MDR phenotype of CML cells that previously exhibit high levels of Pgp/XIAP co-expression.
Keywords:
CML; CSA; Chronic myeloid leukemia; IAPs; IC(50); Imatinib; MDR; MFI; Multidrug resistance; P-glycoprotein; Pgp; RFI; Rho-123; Survivin; VRP; XIAP; chronic myeloid leukemia; concentration achieving 50% inhibition of cell growth; cyclosporin A; inhibitor of apoptosis proteins; mean of fluorescence intensity; multidrug resistance; ratio of mean of fluorescence intensity; rhodamine-123; verapamil.
Copyright © 2013 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics*
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism
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Adolescent
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Adult
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Aged
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Aged, 80 and over
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Antineoplastic Agents / pharmacology*
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Apoptosis / drug effects
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Apoptosis / genetics
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Benzamides / pharmacology*
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Cell Line, Tumor
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Cell Survival / drug effects
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Child
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Drug Resistance, Neoplasm / genetics*
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Female
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Gene Expression Regulation, Leukemic / drug effects
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Gene Expression*
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Gene Knockdown Techniques
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Humans
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Imatinib Mesylate
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Inhibitor of Apoptosis Proteins / genetics
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Inhibitor of Apoptosis Proteins / metabolism
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism
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Male
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Middle Aged
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Piperazines / pharmacology*
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Protein Kinase Inhibitors / pharmacology
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Pyrimidines / pharmacology*
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RNA Interference
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Survivin
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X-Linked Inhibitor of Apoptosis Protein / genetics*
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X-Linked Inhibitor of Apoptosis Protein / metabolism
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Young Adult
Substances
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Antineoplastic Agents
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BIRC5 protein, human
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Benzamides
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Inhibitor of Apoptosis Proteins
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Piperazines
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Protein Kinase Inhibitors
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Pyrimidines
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Survivin
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X-Linked Inhibitor of Apoptosis Protein
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Imatinib Mesylate