The attachment of organelles to the cytoskeleton and directed organelle transport is essential for cellular morphology and function. In contrast to other cell organelles like the endoplasmic reticulum or the Golgi apparatus, peroxisomes are evenly distributed in the cytoplasm, which is achieved by binding of peroxisomes to microtubules and their bidirectional transport by the microtubule motor proteins kinesin-1 (Kif5) and cytoplasmic dynein. KifC3, belonging to the group of C-terminal kinesins, has been identified to interact with the human peroxin PEX1 in a yeast two-hybrid screen. We investigated the potential involvement of KifC3 in peroxisomal transport. Interaction of KifC3 and the AAA-protein (ATPase associated with various cellular activities) PEX1 was confirmed by in vivo colocalization and by coimmunoprecipitation from cell lysates. Furthermore, knockdown of KifC3 using RNAi resulted in an increase of cells with perinuclear-clustered peroxisomes, indicating enhanced minus-end directed motility of peroxisomes. The occurrence of this peroxisomal phenotype was cell cycle phase independent, while microtubules were essential for phenotype formation. We conclude that KifC3 may play a regulatory role in minus-end directed peroxisomal transport for example by blocking the motor function of dynein at peroxisomes. Knockdown of KifC3 would then lead to increased minus-end directed peroxisomal transport and cause the observed peroxisomal clustering at the microtubule-organizing center.
Keywords: 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid; AAA-protein; ATPase associated with diverse cellular activities; CLIP; CoIp; Coimmunoprecipitation; Cytoskeleton; EEA; GAPDH; HEPES; Kif; Kinesins; MAPK; MDCK-II cells; Madin Darby canine kidney II cells; PEX; PMP; PTS-1; Peroxins; Peroxisomes; TGN; cytoplasmic linker protein; early endosomal antigen; glyceraldehyde 3-phosphate dehydrogenase; kinesin superfamily protein; mitogen-activated protein kinase; peroxin; peroxisomal membrane protein; peroxisomal targeting sequence-1; trans-Golgi network.
© 2013.