Sauchinone from Saururus chinensis protects vascular inflammation by heme oxygenase-1 induction in human umbilical vein endothelial cells

Phytomedicine. 2014 Jan 15;21(2):101-8. doi: 10.1016/j.phymed.2013.08.009. Epub 2013 Sep 12.

Abstract

Sauchinone, a diastereomeric lignan isolated from the roots of Saururus chinensis (LOUR.) BAILL. (Saururaceae), is reported to exert a variety of biological activities such as hepatoprotective, anti-inflammatory actions and inhibitory effects on bone resorption. In this study, we investigated the effect of sauchinone in suppressing cell adhesion molecules such as vascular cell adhesion molecule-1 (VCAM-1) and intracellular adhesion molecule-1 (ICAM-1) expression in high glucose stimulated human umbilical vein endothelial cells (HUVEC). Sauchinone inhibited the phosphorylation and degradation of IκB-α, as well as the nuclear translocation of nuclear factor kappa B (NF-κB) p65 caused by the stimulation of high glucose. In addition, sauchinone induced heme oxygenase (HO)-1 expression through nuclear translocation of nuclear factor E2-related factor 2 in HUVEC. The effects of sauchinone on the high glucose-induced expression of VCAM-1 and ICAM-1 and nuclear translocation of NF-κB p65 were partially reversed by transfection of the cells with HO-1 siRNA. These findings suggest that sauchinone-induced HO-1 expression plays a key role in the vascular protective effects of sauchinone in HUVEC.

Keywords: Atherosclerosis; HUVEC; Heme oxygenase-1; NF-κB; Sauchinone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Inflammatory Agents / pharmacology
  • Anti-Inflammatory Agents / therapeutic use*
  • Benzopyrans / pharmacology
  • Benzopyrans / therapeutic use*
  • Dioxoles / pharmacology
  • Dioxoles / therapeutic use*
  • Heme Oxygenase-1 / genetics
  • Heme Oxygenase-1 / metabolism*
  • Human Umbilical Vein Endothelial Cells / drug effects
  • Humans
  • I-kappa B Kinase / metabolism
  • I-kappa B Proteins / metabolism
  • Inflammation / metabolism
  • Inflammation / prevention & control*
  • Intercellular Adhesion Molecule-1 / metabolism
  • NF-E2-Related Factor 2 / metabolism
  • NF-KappaB Inhibitor alpha
  • NF-kappa B / antagonists & inhibitors
  • Phosphorylation
  • Phytotherapy*
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use
  • Saururaceae / chemistry*
  • Transfection
  • Tumor Necrosis Factor-alpha / metabolism
  • Umbilical Veins / cytology
  • Umbilical Veins / drug effects
  • Umbilical Veins / metabolism
  • Vascular Cell Adhesion Molecule-1 / metabolism
  • Vascular Diseases / metabolism
  • Vascular Diseases / prevention & control*

Substances

  • Anti-Inflammatory Agents
  • Benzopyrans
  • Dioxoles
  • I-kappa B Proteins
  • NF-E2-Related Factor 2
  • NF-kappa B
  • NFKBIA protein, human
  • Plant Extracts
  • Tumor Necrosis Factor-alpha
  • Vascular Cell Adhesion Molecule-1
  • sauchinone
  • Intercellular Adhesion Molecule-1
  • NF-KappaB Inhibitor alpha
  • Heme Oxygenase-1
  • I-kappa B Kinase