Abstract
Sauchinone, a diastereomeric lignan isolated from the roots of Saururus chinensis (LOUR.) BAILL. (Saururaceae), is reported to exert a variety of biological activities such as hepatoprotective, anti-inflammatory actions and inhibitory effects on bone resorption. In this study, we investigated the effect of sauchinone in suppressing cell adhesion molecules such as vascular cell adhesion molecule-1 (VCAM-1) and intracellular adhesion molecule-1 (ICAM-1) expression in high glucose stimulated human umbilical vein endothelial cells (HUVEC). Sauchinone inhibited the phosphorylation and degradation of IκB-α, as well as the nuclear translocation of nuclear factor kappa B (NF-κB) p65 caused by the stimulation of high glucose. In addition, sauchinone induced heme oxygenase (HO)-1 expression through nuclear translocation of nuclear factor E2-related factor 2 in HUVEC. The effects of sauchinone on the high glucose-induced expression of VCAM-1 and ICAM-1 and nuclear translocation of NF-κB p65 were partially reversed by transfection of the cells with HO-1 siRNA. These findings suggest that sauchinone-induced HO-1 expression plays a key role in the vascular protective effects of sauchinone in HUVEC.
Keywords:
Atherosclerosis; HUVEC; Heme oxygenase-1; NF-κB; Sauchinone.
Copyright © 2013 Elsevier GmbH. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Anti-Inflammatory Agents / pharmacology
-
Anti-Inflammatory Agents / therapeutic use*
-
Benzopyrans / pharmacology
-
Benzopyrans / therapeutic use*
-
Dioxoles / pharmacology
-
Dioxoles / therapeutic use*
-
Heme Oxygenase-1 / genetics
-
Heme Oxygenase-1 / metabolism*
-
Human Umbilical Vein Endothelial Cells / drug effects
-
Humans
-
I-kappa B Kinase / metabolism
-
I-kappa B Proteins / metabolism
-
Inflammation / metabolism
-
Inflammation / prevention & control*
-
Intercellular Adhesion Molecule-1 / metabolism
-
NF-E2-Related Factor 2 / metabolism
-
NF-KappaB Inhibitor alpha
-
NF-kappa B / antagonists & inhibitors
-
Phosphorylation
-
Phytotherapy*
-
Plant Extracts / pharmacology
-
Plant Extracts / therapeutic use
-
Saururaceae / chemistry*
-
Transfection
-
Tumor Necrosis Factor-alpha / metabolism
-
Umbilical Veins / cytology
-
Umbilical Veins / drug effects
-
Umbilical Veins / metabolism
-
Vascular Cell Adhesion Molecule-1 / metabolism
-
Vascular Diseases / metabolism
-
Vascular Diseases / prevention & control*
Substances
-
Anti-Inflammatory Agents
-
Benzopyrans
-
Dioxoles
-
I-kappa B Proteins
-
NF-E2-Related Factor 2
-
NF-kappa B
-
NFKBIA protein, human
-
Plant Extracts
-
Tumor Necrosis Factor-alpha
-
Vascular Cell Adhesion Molecule-1
-
sauchinone
-
Intercellular Adhesion Molecule-1
-
NF-KappaB Inhibitor alpha
-
Heme Oxygenase-1
-
I-kappa B Kinase