Abstract
The DNA of the gene complementing a PAPS-reductase-deficient strain of Escherichia coli was sequenced. The N-terminal amino acid sequence of the purified PAPS-reductase confirmed that cys H is the structural gene for this enzyme. The open reading frame extends 732 bases and encodes for a peptide of Mr = 27927. The gene product is functionally active when supplemented with thioredoxin and immunologically related with the wild type enzyme.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Blotting, Western
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DNA / analysis*
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Escherichia coli / enzymology*
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Escherichia coli / genetics
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Gene Expression Regulation, Enzymologic*
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Genes, Bacterial*
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Molecular Sequence Data
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Mutation
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Oxidoreductases / analysis
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Oxidoreductases / genetics*
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Plasmids
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Recombinant Proteins / analysis
Substances
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Recombinant Proteins
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DNA
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Oxidoreductases
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3'-phosphoadenylyl-5'-phosphosulfate reductase