Therapeutic effect of Ginkgo biloba polysaccharide in rats with focal cerebral ischemia/reperfusion (I/R) injury

Carbohydr Polym. 2013 Nov 6;98(2):1383-8. doi: 10.1016/j.carbpol.2013.07.045. Epub 2013 Jul 29.

Abstract

An araban type polysaccharide (GBPw) was purified from the leaves of Ginkgo biloba. The present study aimed to investigate the protective effects of GBPw on focal ischemia/reperfusion (I/R) injury in rat brain. The results of this study demonstrated that GBPw had a positive effect on the rat brain when administered 7 days before focal cerebral I/R injury. This effect was evident with the improvements in neurological deficits, reduction in infarct volume, MDA content and the levels of pro-inflammatory cytokines (TNF-α and IL-1β), and elevation in the SOD and MPO activities and the levels of anti-inflammatory cytokine (IL-10). Thus, the beneficial effects of GBPw on cerebral I/R injury may result from the reduction of oxidative stress and the inhibition of NO production and inflammation induced by I/R. The neuroprotective effects of GBPw supplement may have potential implication in the future for prevention/protection against cerebral ischemic stroke.

Keywords: Cerebral ischemia/reperfusional; Ginkgo biloba L.; Neuroprotection; Polysaccharide; Purification.

MeSH terms

  • Animals
  • Brain / blood supply
  • Brain / drug effects
  • Brain / pathology
  • Brain Chemistry
  • Brain Ischemia / drug therapy*
  • Brain Ischemia / metabolism
  • Brain Ischemia / pathology
  • Ginkgo biloba / chemistry*
  • Interleukin-10 / agonists
  • Interleukin-10 / metabolism
  • Interleukin-1beta / antagonists & inhibitors
  • Interleukin-1beta / metabolism
  • Male
  • Malondialdehyde / metabolism
  • Neuroprotective Agents / isolation & purification
  • Neuroprotective Agents / pharmacology*
  • Nitric Oxide / antagonists & inhibitors
  • Nitric Oxide / metabolism
  • Oxidative Stress / drug effects
  • Peroxidase / metabolism
  • Plant Leaves / chemistry*
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology*
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury / drug therapy*
  • Reperfusion Injury / metabolism
  • Reperfusion Injury / pathology
  • Superoxide Dismutase / metabolism
  • Tumor Necrosis Factor-alpha / antagonists & inhibitors
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Interleukin-1beta
  • Neuroprotective Agents
  • Polysaccharides
  • Tumor Necrosis Factor-alpha
  • araban
  • Interleukin-10
  • Nitric Oxide
  • Malondialdehyde
  • Peroxidase
  • Superoxide Dismutase