Interleukin-1 beta in unstimulated whole saliva is a potential biomarker for oral squamous cell carcinoma

T Kamatani; S Shiogama; Y Yoshihama; S Kondo; T Shirota; S Shintani

doi:10.1016/j.cyto.2013.08.011

Interleukin-1 beta in unstimulated whole saliva is a potential biomarker for oral squamous cell carcinoma

Cytokine. 2013 Nov;64(2):497-502. doi: 10.1016/j.cyto.2013.08.011. Epub 2013 Sep 21.

Authors

T Kamatani¹, S Shiogama, Y Yoshihama, S Kondo, T Shirota, S Shintani

Affiliation

¹ Department of Oral & Maxillofacial Surgery, Showa University School of Dentistry, 2-1-1 Kitasenzoku, Ota-City, Tokyo 145-8515, Japan. Electronic address: kamatani@dent.showa-u.ac.jp.

PMID: 24063998
DOI: 10.1016/j.cyto.2013.08.011

Abstract

The objective of this study was to evaluate cytokines in unstimulated whole saliva (UWS) of oral squamous cell carcinoma (OSCC) patients as compared to those with pre- and post-operation for evaluation as markers of OSCC. Sixteen OSCC patients were included in this study. Cytokine concentrations in resting saliva were measured using a Bio-Plex suspension array system. Only interleukin-1 (IL-1) beta showed significantly different cytokine concentration in saliva between pre- and post-operation. IL-1 beta was released from cultured OSCC cells confirmed by ELISA and immunohistochemistry. From this study, IL-1 beta in UWS may be useful for detection of early stage OSCC. More studies are needed to accept the utility of IL-1 beta in UWS for predicting, diagnosis and evaluation of OSCC.

Keywords: Cytokines; Interleukin-1 beta; Oral squamous cell carcinoma; Unstimulated whole saliva.

MeSH terms

Antibodies, Neoplasm / immunology
Biomarkers, Tumor / metabolism*
Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / immunology
Carcinoma, Squamous Cell / metabolism*
Carcinoma, Squamous Cell / pathology
Cell Extracts
Cell Line, Tumor
Epithelium / metabolism
Epithelium / pathology
Female
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Inflammation Mediators / metabolism
Interleukin-1beta / genetics
Interleukin-1beta / metabolism*
Male
Matrix Metalloproteinase 2 / metabolism
Matrix Metalloproteinase 9 / metabolism
Middle Aged
Mouth Neoplasms / genetics
Mouth Neoplasms / immunology
Mouth Neoplasms / metabolism*
Mouth Neoplasms / pathology
RNA, Messenger / genetics
RNA, Messenger / metabolism
Saliva / metabolism*
Staining and Labeling
Vascular Endothelial Growth Factor A / genetics
Vascular Endothelial Growth Factor A / metabolism

Substances

Antibodies, Neoplasm
Biomarkers, Tumor
Cell Extracts
Inflammation Mediators
Interleukin-1beta
RNA, Messenger
Vascular Endothelial Growth Factor A
MMP2 protein, human
Matrix Metalloproteinase 2
MMP9 protein, human
Matrix Metalloproteinase 9