End stage renal disease is a clinical state that extends from chronic renal failure and is marked by an irreversible loss of renal function. TGF-β1 mediated renal fibrosis is a common pathology implicated in this form of kidney disease. In this study circulating protein and mRNA levels of TGF-β1 cytokine were investigated among ESRD patients and respective controls from North India. Physician diagnosed 192 ESRD patients, on hemodialysis, and 130 normal controls participated in the present study. TGF-β1 circulating levels were measured by ELISA and its expression was quantified using competitive-PCR. Mean TGF-β1 protein levels were 2.7-fold lower in ESRD patients as compared to normal controls (p<0.001). Additionally, TGF-β1 mRNA transcripts of this cytokine were also significantly lower in the diseased population compared to controls (p<0.001). These results imply that TGF-β1 has not played its anticipated pro-fibrotic role and anti-inflammatory function in the studied population.
Keywords: ANOVA; C-PCR; Cytokine; ELISA; ESRD; Hemodialysis; PCR; TGF-β(1); analysis of variance; competitive polymerase chain reaction; end stage renal disease; enzyme linked immunosorbent assay; mRNA; polymerase chain reaction; transforming growth factor beta 1.
© 2013.