Immunoglobulin heavy chain gene rearrangement in oral B cell lymphomas

Maria Fernanda Setubal Destro Rodrigues; Ricardo Alves Mesquita; Lília Alves Rocha; Fabio Daumas Nunes; Suzana Cantanhede Orsini Machado de Sousa

doi:10.1016/j.oooo.2013.07.007

Immunoglobulin heavy chain gene rearrangement in oral B cell lymphomas

Oral Surg Oral Med Oral Pathol Oral Radiol. 2013 Nov;116(5):607-13. doi: 10.1016/j.oooo.2013.07.007.

Authors

Maria Fernanda Setubal Destro Rodrigues¹, Ricardo Alves Mesquita, Lília Alves Rocha, Fabio Daumas Nunes, Suzana Cantanhede Orsini Machado de Sousa

Affiliation

¹ Department of Oral Pathology, Dental School, University of São Paulo, São Paulo, São Paulo, Brazil.

PMID: 24119524
DOI: 10.1016/j.oooo.2013.07.007

Abstract

Objective: Oral non-Hodgkin lymphomas (NHLs) are an extensive group of malignant lymphoid cell neoplasms that are the second most common group of oral cancers. Subtyping NHL is important to plan for appropriate treatment, and the analysis of clonality is in many instances used for helping in the diagnosis of NHL. Thus, the aim of this study was to analyze immunoglobulin heavy chain (IgH) gene rearrangement in a series of oral B cell lymphomas to investigate the sensitivity of seminested polymerase chain reaction (snPCR).

Study design: Paraffin embedded tissue samples from 16 cases of oral B cell lymphomas were retrieved and subjected to snPCR to investigate the IgH gene rearrangement.

Results: The results showed monoclonal IgH rearrangement in 85.7% of the cases studied, as represented by finding one band within the expected range of amplification.

Conclusions: This study found that snPCR is a consistent method for the detection of gene rearrangement in paraffin-embedded tissue.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Female
Gene Rearrangement, B-Lymphocyte, Heavy Chain*
Humans
Immunohistochemistry
Lymphoma, B-Cell / genetics*
Male
Middle Aged
Mouth Neoplasms / genetics*
Paraffin Embedding
Polymerase Chain Reaction