miR-15a inhibits cell proliferation and epithelial to mesenchymal transition in pancreatic ductal adenocarcinoma by down-regulating Bmi-1 expression

Cancer Lett. 2014 Mar 1;344(1):40-46. doi: 10.1016/j.canlet.2013.10.009. Epub 2013 Nov 16.

Abstract

Purposes: To investigate whether miR-15a inhibits cell proliferation and epithelial-mesenchymal transition (EMT) in pancreatic ductal adenocarcinoma (PDAC) via the down-regulation of B cell-specific moloney murine leukemia virus insertion site 1 (Bmi-1) expression.

Methods and results: miR-15a and Bmi-1 expressions in normal pancreatic tissue and PDAC tissue were measured. The relationship between miR-15a and Bmi-1 expression was analyzed. We found that miR-15a suppressed the expression of Bmi-1 and PDAC cell proliferation; E-cadherin expression was visibly up-regulated after silencing Bmi-1 by transfecting miR-15a into PDAC cell line.

Conclusion: miR-15a inhibits cell proliferation and EMT in PDAC via the down-regulation of Bmi-1 expression.

Keywords: Bmi-1; EMT; Pancreatic ductal adenocarcinoma; Proliferation; miR-15a.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma, Pancreatic Ductal / genetics*
  • Carcinoma, Pancreatic Ductal / metabolism
  • Cell Line, Tumor
  • Cell Proliferation*
  • Down-Regulation
  • Epithelial-Mesenchymal Transition / physiology*
  • Female
  • Gene Expression Regulation, Neoplastic / physiology*
  • Humans
  • Immunohistochemistry
  • Male
  • MicroRNAs / genetics*
  • Middle Aged
  • Pancreatic Neoplasms / genetics*
  • Pancreatic Neoplasms / metabolism
  • Polycomb Repressive Complex 1 / biosynthesis*
  • Real-Time Polymerase Chain Reaction
  • Transfection

Substances

  • BMI1 protein, human
  • MIRN15 microRNA, human
  • MicroRNAs
  • Polycomb Repressive Complex 1