Hsa-let-7g miRNA targets caspase-3 and inhibits the apoptosis induced by ox-LDL in endothelial cells

Int J Mol Sci. 2013 Nov 18;14(11):22708-20. doi: 10.3390/ijms141122708.

Abstract

It has been well confirmed ox-LDL plays key roles in the development of atherosclerosis via binding to LOX-1 and inducing apoptosis in vascular endothelial cells. Recent studies have shown ox-LDL can suppress microRNA has-let-7g, which in turn inhibits the ox-LDL induced apoptosis. However, details need to be uncovered. To determine the anti-atherosclerosis effect of microRNA has-let-7g, and to evaluate the possibility of CASP3 as an anti-atherosclerotic drug target by has-let-7g, the present study determined the role of hsa-let-7g miRNA in ox-LDL induced apoptosis in the vascular endothelial cells. We found that miRNA has-let-7g was suppressed during the ox-LDL-induced apoptosis in EAhy926 endothelial cells. In addition, overexpression of has-let-7g negatively regulated apoptosis in the endothelial cells by targeting caspase-3 expression. Therefore, miRNA let-7g may play important role in endothelial apoptosis and atherosclerosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / genetics
  • Atherosclerosis / drug therapy
  • Atherosclerosis / genetics*
  • Atherosclerosis / pathology
  • Caspase 3 / biosynthesis*
  • Caspase 3 / genetics
  • Cell Line
  • Cell Survival / genetics
  • Endothelial Cells / metabolism
  • Gene Expression Regulation / genetics
  • Humans
  • Lipoproteins, LDL / antagonists & inhibitors
  • Lipoproteins, LDL / metabolism*
  • MicroRNAs / genetics*
  • Signal Transduction

Substances

  • Lipoproteins, LDL
  • MicroRNAs
  • mirnlet7 microRNA, human
  • oxidized low density lipoprotein
  • Caspase 3