miR-181b promotes cell proliferation and reduces apoptosis by repressing the expression of adenylyl cyclase 9 (AC9) in cervical cancer cells

FEBS Lett. 2014 Jan 3;588(1):124-30. doi: 10.1016/j.febslet.2013.11.019. Epub 2013 Nov 20.

Abstract

MicroRNAs are a class of small, endogenous, non-coding RNAs that function as post-transcriptional regulators. In this study, we found that miR-181b promoted cell proliferation and inhibited cell apoptosis in cervical cancer cells. And we validated a new miR-181b target gene, adenylyl cyclase 9 (AC9). miR-181b restricted cAMP production by post-transcriptionally downregulating AC9 expression. Phenotypic experiments indicated that miR-181b and AC9 exerted opposite effects on cell proliferation and apoptosis.

Keywords: AC9; ASO; Adenylyl cyclase 9; Cervical cancer cell; EGFP; GAPDH; TUNEL; UTR; adenylyl cyclase 9; antisense oligonucleotide; cAMP; enhanced green fluorescence protein; glyceraldehyde-3-phosphate dehydrogenase; miR-181b; miRNA; microRNA; microRNA-181b; terminal deoxynucleotidyl transferase dUTP nick end labeling; untranslated region.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions / genetics
  • Adenylyl Cyclases / genetics*
  • Adenylyl Cyclases / metabolism
  • Apoptosis / genetics*
  • Base Sequence
  • Binding Sites / genetics
  • Blotting, Western
  • Cell Line, Tumor
  • Cell Proliferation*
  • Cell Survival / genetics
  • Cyclic AMP / metabolism
  • Female
  • Gene Expression Regulation, Neoplastic
  • HeLa Cells
  • Humans
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Oligonucleotides, Antisense / genetics
  • RNA Interference
  • Reverse Transcriptase Polymerase Chain Reaction
  • Uterine Cervical Neoplasms / genetics*
  • Uterine Cervical Neoplasms / metabolism
  • Uterine Cervical Neoplasms / pathology

Substances

  • 3' Untranslated Regions
  • MIrn181 microRNA, human
  • MicroRNAs
  • Oligonucleotides, Antisense
  • Cyclic AMP
  • Adenylyl Cyclases
  • adenylate cyclase 9