Abstract
Background:
Breast cancer is a major health problem worldwide. Olive oil induces apoptosis in some cancer cells due to phenolic compounds like oleuropein. Although oleuropein has anticancer activity, the underlying mechanisms of action remain unknown. The study aimed to assess the mechanism of oleuropin-induced breast cancer cell apoptosis.
Materials and methods:
p53, Bcl-2 and Bax gene expression was evaluated by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) in luminal MCF-7 cells.
Results:
Oleuropein-induced apoptosis was accompanied by up-regulation of both p53 and Bax gene expression levels and down-regulation in Bcl2.
Conclusions:
Oleuropein induces apoptosis in breast tumour cells via a p53-dependent pathway mediated by Bax and Bcl2 genes. Therefore, oleuropein may have therapeutic potential in breast cancer patients by inducing apoptosis via activation of the p53 pathway.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antihypertensive Agents / pharmacology*
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Blotting, Western
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Breast Neoplasms / drug therapy*
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Breast Neoplasms / genetics
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Breast Neoplasms / pathology*
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Cell Proliferation / drug effects
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Female
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Gene Expression Regulation, Neoplastic / drug effects*
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Humans
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Iridoid Glucosides
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Iridoids / pharmacology*
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Proto-Oncogene Proteins c-bcl-2 / genetics*
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Proto-Oncogene Proteins c-bcl-2 / metabolism
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RNA, Messenger / genetics
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Real-Time Polymerase Chain Reaction
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Reverse Transcriptase Polymerase Chain Reaction
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Tumor Cells, Cultured
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Tumor Suppressor Protein p53 / genetics*
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Tumor Suppressor Protein p53 / metabolism
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bcl-2-Associated X Protein / genetics*
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bcl-2-Associated X Protein / metabolism
Substances
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Antihypertensive Agents
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BAX protein, human
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Iridoid Glucosides
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Iridoids
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Proto-Oncogene Proteins c-bcl-2
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RNA, Messenger
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TP53 protein, human
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Tumor Suppressor Protein p53
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bcl-2-Associated X Protein
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oleuropein