Atp6v1c1 may regulate filament actin arrangement in breast cancer cells

Ming Cai; Pengcheng Liu; Li Wei; Jinshen Wang; Jin Qi; Shengmei Feng; Lianfu Deng

doi:10.1371/journal.pone.0084833

Atp6v1c1 may regulate filament actin arrangement in breast cancer cells

PLoS One. 2014 Jan 15;9(1):e84833. doi: 10.1371/journal.pone.0084833. eCollection 2014.

Authors

Ming Cai¹, Pengcheng Liu², Li Wei¹, Jinshen Wang¹, Jin Qi¹, Shengmei Feng, Lianfu Deng

Affiliations

¹ Shanghai Institute of Traumatology and Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases with Integrated Chinese-Western Medicine, Ruijin Hospital, Jiao Tong University School of Medicine, Shanghai, China.
² Department of Orthopaedics, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.

Abstract

Previous studies have shown that the rate of breast cancer metastasis correlates with the expression of vacuolar H(+)-ATPases (V-ATPases). However, how V-ATPase is involved in breast cancer metastasis remains unknown. Our previous study showed that Atp6v1c1-depleted osteoclasts did not form organized actin rings and that Atp6v1c1 co-localizes with F-actin. In this study, we found that the normal arrangement of filamentous actin is disrupted in Atp6v1c1-depleted 4T1 mouse breast cancer cells and in the ATP6V1C1-depleted human breast cancer cell lines MDA-MB-231 and MDA-MB-435s. We further found that Atp6v1c1 co-localizes with F-actin in 4T1 cells. The results of our study suggest that high expression of Atp6v1c1 affects the actin structure of cancer cells such that it facilitates breast cancer metastasis. The findings also indicate that Atp6v1c1 could be a novel target for breast cancer metastasis therapy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actins / metabolism*
Animals
Breast Neoplasms / metabolism*
Breast Neoplasms / pathology
Cell Line, Tumor
Female
Gene Knockdown Techniques
Humans
Mice
Reverse Transcriptase Polymerase Chain Reaction
Vacuolar Proton-Translocating ATPases / genetics
Vacuolar Proton-Translocating ATPases / physiology*

Substances

Actins
ATP6V1C1 protein, human
Vacuolar Proton-Translocating ATPases

Grants and funding

This work was supported by the National Natural Science Foundation of China (Grant 30901525), Shanghai Natural Science Foundation (Grant 09ZR1428900), Shanghai Education Commission Foundation (Grant 10YZ39), Talents Cultivation Plan of Shanghai Health System (XYQ2011050), and a Science and Technology Grant from the Medical School of Shanghai, Jiaotong University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.