MiR-26a promotes ovarian cancer proliferation and tumorigenesis

Wenjing Shen; Min Song; Jie Liu; Guangrong Qiu; Tianren Li; Yanjie Hu; Hongbo Liu

doi:10.1371/journal.pone.0086871

MiR-26a promotes ovarian cancer proliferation and tumorigenesis

PLoS One. 2014 Jan 22;9(1):e86871. doi: 10.1371/journal.pone.0086871. eCollection 2014.

Authors

Wenjing Shen¹, Min Song², Jie Liu³, Guangrong Qiu⁴, Tianren Li¹, Yanjie Hu¹, Hongbo Liu⁵

Affiliations

¹ Department of Gynecology, The First Affiliated Hospital of China Medical University, Shenyang, China.
² Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Institute of Pathology and Pathophysiology, Shenyang, China.
³ Experimental Technology Center, China Medical University, Shenyang, China.
⁴ Department of Medical Genetics, China Medical University, Shenyang, China.
⁵ Department of Health Statistics, School of Public Health, China Medical University, Shenyang, China.

Abstract

MicroRNAs (miRNAs) important for posttranscriptional gene expression are involved in the initiation and progression of human cancer. In this study, we reported that miR-26a was over-expressed in human EOC specimens and the expression level of extracellular miR-26a in plasma can distinguish patients from healthy controls in EOC. Ectopic expression of miR-26a in ovarian cancer (OC) cells increased cell proliferation and clonal formation. This growth promoting effect of OC cell growth was mediated by miR-26a inhibition of the posttranscription of ER-α. Furthermore, inhibition of miR-26a suppressed the tumor formation generated by injecting OC cells in nude mice. Our results suggest that aberrantly expressed miR-26a may contribute to OC development.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blotting, Western
Carcinogenesis / metabolism*
Cell Line, Tumor
Cell Proliferation*
Cloning, Molecular
Female
Humans
Luciferases
Mice
Mice, Nude
MicroRNAs / metabolism*
Ovarian Neoplasms / genetics
Ovarian Neoplasms / physiopathology*
Real-Time Polymerase Chain Reaction
Statistics, Nonparametric

Substances

MIRN26A microRNA, human
MicroRNAs
Luciferases

Grants and funding

This work was sponsored by grants from the Project No. 30571836 from the NNSF(National Natural Science Foundation) of China. In addition, the work presented is part of projects with Project No.L2010681 financially supported by Science Foundation of the Education Department of Liaoning Province of China, and Project No.201202259 financially supported by Science Foundation of Science and Technology Bureau of Liaoning Province of China. Author Min Song contributed to experimental conceiving and design, guidance and discussion of the project, so she is the co-correspondence author of this paper. All the other funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.