miRNA423-5p regulates cell proliferation and invasion by targeting trefoil factor 1 in gastric cancer cells

Cancer Lett. 2014 May 28;347(1):98-104. doi: 10.1016/j.canlet.2014.01.024. Epub 2014 Jan 31.

Abstract

TFF1 is a small, secreted protein in the TFF family that has a pivotal role as a motogenic factor in epithelial restitution and cell motility, and as a tumor suppressor gene in the stomach. In this study, we identified TFF1 as a novel target gene of miRNA-423-5p. miRNA-423-5p negatively regulated the expression of TFF1 by binding to its 3'UTR and participated in proliferation/invasion-related processes via a TFF1-dependent manner in gastric cancer cells. Our findings suggested that miR-423-5p may be a novel target for the future development of specific therapeutic interventions for gastric cancer.

Keywords: Expression regulation; Gastric cancer; Proliferation and invasion; Trefoil factor 1 (TFF1); miR-423-5p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Line, Tumor
  • Cell Proliferation*
  • Cyclin D / metabolism
  • DNA Primers
  • Humans
  • MicroRNAs / physiology*
  • Neoplasm Invasiveness*
  • Polymerase Chain Reaction
  • Stomach Neoplasms / genetics
  • Stomach Neoplasms / metabolism
  • Stomach Neoplasms / pathology*
  • Trefoil Factor-1
  • Tumor Suppressor Proteins / physiology*
  • beta Catenin / metabolism

Substances

  • Cyclin D
  • DNA Primers
  • MIRN423 microRNA, human
  • MicroRNAs
  • TFF1 protein, human
  • Trefoil Factor-1
  • Tumor Suppressor Proteins
  • beta Catenin