Kaiso interacts with p120-catenin to regulate β-catenin expression at the transcriptional level

Yang Liu; Qian-Ze Dong; Si Wang; Hong-Tao Xu; Yuan Miao; Liang Wang; En-Hua Wang

doi:10.1371/journal.pone.0087537

Kaiso interacts with p120-catenin to regulate β-catenin expression at the transcriptional level

PLoS One. 2014 Feb 3;9(2):e87537. doi: 10.1371/journal.pone.0087537. eCollection 2014.

Authors

Yang Liu¹, Qian-Ze Dong¹, Si Wang², Hong-Tao Xu¹, Yuan Miao¹, Liang Wang¹, En-Hua Wang¹

Affiliations

¹ Department of Pathology, the First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, PR China.
² Department of Medical Microbiology and Parasitology, College of Basic Medical Sciences of China Medical University, Shenyang, PR China.

Abstract

Background: We have reported that p120-catenin could regulate β-catenin transcription in lung cancer cells, but the specific mechanism is unclear.

Methods and results: In this study, bisulfite sequencing PCR showed that the β-catenin promoter region in SPC-A-1 and LTEP-a-2 lung cancer cell lines has Kaiso binding sites sequences and CpG islands which may combine with Kaiso. The demethylating reagent 5-Aza-2'-deoxycytidine significantly upregulated β-catenin mRNA expression in lung cancer cell lines, whereas expression was significantly reduced following transfection with Kaiso. However, the upregulation of β-catenin mRNA expression after treatment with 5-Aza-2'-deoxycytidine was not reduced by subsequent transfection with Kaiso cDNA. Chromatin immunoprecipitation showed that, in lung cancer cell lines, methylated CpG-dinucleotides sequences combined with Kaiso and the Kaiso binding sites sequence did not. The capacity of Kaiso to combine with p120-catenin isoforms was confirmed by immunoprecipitation.

Conclusions: Based on these results, we concluded that Kaiso participates in the regulation by p120ctn of β-catenin mRNA expression in the lung cancer cell lines.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Azacitidine / analogs & derivatives
Azacitidine / pharmacology
Blotting, Western
Catenins / genetics*
Catenins / metabolism
Cell Line, Tumor
CpG Islands / genetics
DNA Methylation
Decitabine
Delta Catenin
Enzyme Inhibitors / pharmacology
Gene Expression Regulation, Neoplastic / drug effects
Humans
Lung Neoplasms / genetics
Lung Neoplasms / metabolism
Lung Neoplasms / pathology
Promoter Regions, Genetic / genetics
Protein Binding
Reverse Transcriptase Polymerase Chain Reaction
Transcription Factors / genetics*
Transcription Factors / metabolism
Transcription, Genetic*
Up-Regulation / drug effects
beta Catenin / genetics*
beta Catenin / metabolism

Substances

Catenins
Enzyme Inhibitors
Transcription Factors
ZBTB33 protein, human
beta Catenin
Decitabine
Azacitidine
Delta Catenin
CTNND1 protein, human

Grants and funding

This work was supported by grants from the National Natural Science Foundation of China (No. 30900562 to YL, No. 81000942 to YM) and the Ph.D. Programs Foundation of Ministry of Education of China (No. 20092104120022 to YL). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.