C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells

Branko Zevnik; Nathalie C Uyttersprot; Ana V Perez; Gerald W M Bothe; Heidrun Kern; Gunther Kauselmann

doi:10.1371/journal.pone.0090570

C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells

PLoS One. 2014 Mar 5;9(3):e90570. doi: 10.1371/journal.pone.0090570. eCollection 2014.

Authors

Branko Zevnik¹, Nathalie C Uyttersprot², Ana V Perez³, Gerald W M Bothe³, Heidrun Kern², Gunther Kauselmann²

Affiliations

¹ Cologne Excellence Cluster on Cellular Stress Responses in Aging-associated Diseases (CECAD), University of Cologne, Cologne, Germany.
² TaconicArtemis GmbH, Cologne, Germany.
³ Taconic, Hudson, New York, United States of America.

Abstract

We generated C57BL/6NTac mice carrying a tyrosinase loss-of function mutation and a reversion of the nonagouti locus to agouti. This strain has a high superovulation response, allows visual detection of chimeric coat color contribution of C57BL/6 ES-cells and provides a simplified breeding format that generates black G1 offspring of pure inbred C57BL/6 background in one step, providing the ideal host for genetically manipulated C57BL/6 ES cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agouti Signaling Protein / genetics*
Animals
Base Sequence
Cells, Cultured
Chimera / genetics
Embryo Transfer
Embryonic Stem Cells / physiology*
Female
Genetic Loci
Hair Color / genetics
Male
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Transgenic
Monophenol Monooxygenase / genetics
Retroelements
Superovulation

Substances

Agouti Signaling Protein
Retroelements
a protein, mouse
Monophenol Monooxygenase

Grants and funding

This work has been funded by Taconic (NY, USA). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.