The XAGE-1b gene has been identified in numerous malignancies in the human body. However, little is known regarding its mechanism for promoting tumorigenesis in adenoid cystic carcinoma. The aim of this study was to explore the correlation between tumor cell growth and the XAGE-1b gene. The constructed PCMV-Myc plasmid vector containing the XAGE-1b gene and transfected adenoid cystic carcinoma (ACC)-2 cells was applied to study cell cycle alterations and anti-apoptotic effects. These were assessed by flow cytometry with PI staining and the measurement of cell content at its Sub-G1 phase, respectively. The fluorescence intensity representing the regulation of XAGE-1b on the transcription factors located downstream of the signaling pathway using the Mercury pathway profiling system was also detected. XAGE-1b over expression promoted cell growth by shortening G0-G1 and prolonging the G2-M phase. Additionally, XAGE-1b overexpression enhanced the anti-apoptotic effects induced by tumor necrosis factor-α (TNF-α) and serum deprivation in ACC-2 cells. The results of the present study suggested that XAGE-1b gene is crucial in the tumorigenesis of ACC, and its mechanism should be further investigated.
Keywords: XAGE-1b gene; adenoid cystic carcinoma; apoptosis; cancer testis antigen; signaling pathway; tumor necrosis factor-α.