Mir-23b and miR-130b expression is downregulated in pituitary adenomas

Vincenza Leone; Concetta Langella; Daniela D'Angelo; Paula Mussnich; Anne Wierinckx; Luigi Terracciano; Gerald Raverot; Joel Lachuer; Sandra Rotondi; Marie-Lise Jaffrain-Rea; Jacqueline Trouillas; Alfredo Fusco

doi:10.1016/j.mce.2014.03.002

Mir-23b and miR-130b expression is downregulated in pituitary adenomas

Mol Cell Endocrinol. 2014 Jun 5;390(1-2):1-7. doi: 10.1016/j.mce.2014.03.002. Epub 2014 Mar 28.

Affiliations

¹ Istituto di Endocrinologia ed Oncologia Sperimentale del CNR, c/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Scuola di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli "Federico II", Naples, Italy.
² INSERM U1052, Centre de Recherche en Cancerologie de Lyon, F-69000 Lyon, France and University Lyon, F-69000 Lyon, France; Profilexpert UNIV-US7 INSERM-UMS 3453 CNRS Lyon, France.
³ Molecular Pathology Division Institute of Pathology, University HospitalSchönbeinstrasse, 40CH-4003 Basel, Switzerland.
⁴ INSERM U1028, CNRS UMR 5292, Lyon Neuroscience Research Center, Neuro-oncology and Neuroinflammation Team, F-69372 Lyon Cedex 08, France and University Lyon1, F-69000 Lyon, France.
⁵ Profilexpert UNIV-US7 INSERM-UMS 3453 CNRS Lyon, France.
⁶ Department ofClinical Applied Sciencesand Biotechnology, University of L'Aquila Via Vetoio, Coppito 2, 67100 L'Aquila, Italy.
⁷ Department ofClinical Applied Sciencesand Biotechnology, University of L'Aquila Via Vetoio, Coppito 2, 67100 L'Aquila, Italy; Neuromed, IRCCS, Pozzilli, Italy.
⁸ INSERM U1052, Centre de Recherche en Cancerologie de Lyon, F-69000 Lyon, France and University Lyon, F-69000 Lyon, France; INSERM U1028, CNRS UMR 5292, Lyon Neuroscience Research Center, Neuro-oncology and Neuroinflammation Team, F-69372 Lyon Cedex 08, France and University Lyon1, F-69000 Lyon, France.
⁹ Istituto di Endocrinologia ed Oncologia Sperimentale del CNR, c/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Scuola di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli "Federico II", Naples, Italy. Electronic address: alfusco@unina.it.

PMID: 24681352
DOI: 10.1016/j.mce.2014.03.002

Abstract

MicroRNA (miRNA) deregulation plays a critical role in tumorigenesis. miR-23b and miR-130b are induced by thyrotropin in thyroid cells in a cAMP-dependent manner. The aim of our work has been to investigate the possible role of miR-23b and miR-130b in pituitary tumorigenesis. We have analyzed their expression in a panel of pituitary adenomas (PAs) including GH and NFPA adenomas. We report that miR-23b and miR-130b are drastically reduced in GH, gonadotroph and NFPA adenomas in comparison with normal pituitary gland. Interestingly, the overexpression of miR-23b and miR-130b inhibits cell proliferation arresting the cells in the G1 and G2 phase of the cell cycle, respectively. Moreover, we demonstrate that miR-23b and miR-130b target HMGA2 and cyclin A2 (CCNA2) genes, respectively. Finally, downregulation of miR-23b and miR-130b expression is associated with increased levels of their respective targets in human PAs. These findings suggest that miR-23b and miR-130b downregulation may contribute to pituitary tumorigenesis.

Keywords: CCNA2; HMGA2; Pituitary adenoma; microRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3' Untranslated Regions
Adenoma / genetics
Adenoma / metabolism*
Animals
Binding Sites
Carcinogenesis / genetics
Carcinogenesis / metabolism
Case-Control Studies
Cell Line, Tumor
Cell Proliferation
Down-Regulation
Gene Expression Regulation, Neoplastic
Gene Expression*
HEK293 Cells
HMGA2 Protein / genetics
HMGA2 Protein / metabolism
Humans
MicroRNAs / genetics
MicroRNAs / metabolism*
Pituitary Gland / metabolism
Pituitary Gland / pathology
Pituitary Neoplasms / genetics
Pituitary Neoplasms / metabolism*
RNA Interference
Rats

Substances

3' Untranslated Regions
HMGA2 Protein
MIRN130 microRNA, human
MIRN23a microRNA, human
MicroRNAs