The expression of LEP, LEPR, IGF1 and IL10 in obesity and the relationship with microRNAs

Renata Viesti A Collares; Wilson Salgado Jr; Daniela Pretti da Cunha Tirapelli; José Sebastião dos Santos

doi:10.1371/journal.pone.0093512

The expression of LEP, LEPR, IGF1 and IL10 in obesity and the relationship with microRNAs

PLoS One. 2014 Apr 1;9(4):e93512. doi: 10.1371/journal.pone.0093512. eCollection 2014.

Authors

Renata Viesti A Collares¹, Wilson Salgado Jr², Daniela Pretti da Cunha Tirapelli³, José Sebastião dos Santos²

Affiliations

¹ Department of Surgery and Anatomy of the Faculty of Medicine of Ribeirao Preto, University of São Paulo, Ribeirao Preto, SP, Brazil.
² Department of Surgery and Anatomy of the Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirao Preto, SP, Brazil.
³ Department of Surgery and Anatomy of the Faculty of Medicine of Ribeirão Preto, University of São Paulo and Laboratory of Molecular Biology, Ribeirao Preto, SP, Brazil.

Abstract

Obesity is a multifactorial disease, with epigenetic alterations. Have been described modifications in the expression of some microRNAs, and some proteins related to obesity. The objective was to determine and correlate, in obese patients, the gene expression of LEP, LEPR, IGF1, IL10 and of miR-27a, miR-27b, miR-143 and miR-145. RNA was extracted from biopsies of subcutaneous fat, liver and visceral fat of 15 obese subjects submitted to bariatric surgery and of 15 non-obese subjects submitted to cholecystectomy for cDNA synthesis and for RT-PCR. The microRNAs were chosen using the TargetScan software. An increased expression of LEP and IGF1 was detected in the subcutaneous fat of the obese group compared to control, while the expression of IGF1 was higher in the control group than in the obese one. MiRNA-27a had a higher expression in the omentum of the obese patients and there was also a correlation in the expression of miRNA-145 and LEPR in the omentum of this group.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Case-Control Studies
Female
Gene Expression Profiling
Gene Expression Regulation
Gene Expression*
Humans
Insulin-Like Growth Factor I / genetics*
Interleukin-10 / genetics*
Leptin / genetics*
Male
MicroRNAs / genetics*
Middle Aged
Obesity / genetics*
Receptors, Leptin / genetics*
Young Adult

Substances

Leptin
MicroRNAs
Receptors, Leptin
Interleukin-10
Insulin-Like Growth Factor I

Grants and funding

Research supported by Fundação Waldemar Barnsley Pessoa, Ribeirão Preto, SP, Brazil. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.