Abstract
tat, the trans-activator protein for human immunodeficiency virus 1 (HIV-1), has been expressed in Escherichia coli from synthetic genes. Purified tat binds specifically to HIV-1 trans-activation-responsive region (TAR) RNA in gel-retardation, filter-binding, and immunoprecipitation assays. tat does not bind detectably to antisense TAR RNA sequences, cellular mRNA sequences, variant TAR RNA sequences with altered stem-loop structures, or TAR DNA.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Escherichia coli / genetics
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Gene Products, tat
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Genes
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Genes, Synthetic
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Genes, Viral
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HIV-1 / genetics
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HIV-1 / metabolism*
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HeLa Cells / metabolism
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Humans
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Kinetics
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Molecular Sequence Data
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Nucleic Acid Conformation
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Oncogene Proteins, Viral / metabolism
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Plasmids
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Protein Binding
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RNA / metabolism*
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RNA, Antisense
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Transcription Factors / genetics
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Transcription Factors / isolation & purification
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Transcription Factors / metabolism*
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Transcription, Genetic
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tat Gene Products, Human Immunodeficiency Virus
Substances
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Gene Products, tat
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Oncogene Proteins, Viral
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RNA, Antisense
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Recombinant Proteins
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Transcription Factors
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tat Gene Products, Human Immunodeficiency Virus
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RNA