p21 rs3176352 G>C and p73 rs1801173 C>T polymorphisms are associated with an increased risk of esophageal cancer in a Chinese population

Liang Zheng; Weifeng Tang; Yijun Shi; Suocheng Chen; Xu Wang; Liming Wang; Aizhong Shao; Guowen Ding; Chao Liu; Ruiping Liu; Jun Yin; Haiyong Gu

doi:10.1371/journal.pone.0096958

p21 rs3176352 G>C and p73 rs1801173 C>T polymorphisms are associated with an increased risk of esophageal cancer in a Chinese population

PLoS One. 2014 May 12;9(5):e96958. doi: 10.1371/journal.pone.0096958. eCollection 2014.

Authors

Liang Zheng¹, Weifeng Tang², Yijun Shi², Suocheng Chen², Xu Wang², Liming Wang³, Aizhong Shao², Guowen Ding², Chao Liu², Ruiping Liu⁴, Jun Yin², Haiyong Gu²

Affiliations

¹ Department of Cardiothoracic Surgery, The First People's Hospital of Changzhou and The Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu Province, China.
² Department of Cardiothoracic Surgery, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, China.
³ Cancer institute, Department of chemotherapy, People's Hospital Affiliated to Jiangsu University, Zhenjiang, Jiangsu Province, China.
⁴ Department of Orthopedics, Affiliated Hospital of Nanjing Medical University, Changzhou Second People's Hospital, Changzhou, Jiangsu Province, China.

Abstract

Objective: Esophageal cancer was the fifth most commonly diagnosed cancer and the fourth leading cause of cancer-related death in China in 2009. Genetic factors might play an important role in esophageal squamous cell carcinoma (ESCC) carcinogenesis.

Designs and methods: To evaluate the effect p21, p53, TP53BP1 and p73 single nucleotide polymorphisms (SNPs) on the risk of ESCC, we conducted a hospital based case-control study. A total of 629 ESCC cases and 686 controls were recruited. Their genotypes were determined using ligation detection reaction (LDR) method.

Results: When the p21 rs3176352 GG homozygote genotype was used as the reference group, the CC genotype was associated with a significantly increased risk of ESCC. When the p73 rs1801173 CC homozygote genotype was used as the reference group, the CT genotype was associated with a significantly increased risk of ESCC. After Bonferroni correction, for p21 rs3176352 G>C, the pcorrect was still significant. For the other six SNPs, in all comparison models, no association between the polymorphisms and ESCC risk was observed.

Conclusions: p21 rs3176352 G>C and p73 rs1801173 C>T SNPs are associated with increased risk of ESCC. To confirm the current findings, additional, larger studies and tissue-specific biological characterization are required.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Asian People / genetics*
Carcinoma, Squamous Cell / genetics*
Case-Control Studies
Cyclin-Dependent Kinase Inhibitor p21 / genetics*
DNA-Binding Proteins / genetics
Esophageal Neoplasms / genetics*
Esophageal Squamous Cell Carcinoma
Female
Genetic Predisposition to Disease / genetics
Genotyping Techniques
Humans
Intracellular Signaling Peptides and Proteins / genetics
Male
Middle Aged
Nuclear Proteins / genetics
Polymorphism, Single Nucleotide / genetics*
Tumor Protein p73
Tumor Suppressor Protein p53 / genetics
Tumor Suppressor Proteins / genetics
Tumor Suppressor p53-Binding Protein 1

Substances

Cyclin-Dependent Kinase Inhibitor p21
DNA-Binding Proteins
Intracellular Signaling Peptides and Proteins
Nuclear Proteins
TP53BP1 protein, human
TP73 protein, human
Tumor Protein p73
Tumor Suppressor Protein p53
Tumor Suppressor Proteins
Tumor Suppressor p53-Binding Protein 1

Grants and funding

This study was supported in part by National Natural Science Foundation of China (81370001, 81371927, 81101889, 81000028), Jiangsu Province Natural Science Foundation (BK2010333, BK2011481), Social Development Foundation of Zhenjiang (SH2010017) and Changzhou Young Talents and Science-Technology Foundation of Health Bureau (QN201102) and Affiliated People's Hospital of Jiangsu University fund (Y200913). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.