APP-695 transcript prevalence is selectively reduced during Alzheimer's disease in cortex and hippocampus but not in cerebellum

Neurobiol Aging. 1989 Nov-Dec;10(6):755-60. doi: 10.1016/0197-4580(89)90017-1.

Abstract

We previously found a selective loss of the brain specific APP-695 transcript in Alzheimer's disease (AD) cortex, which results in a 2.5-fold increase in the APP-751/APP-695 transcript ratio in AD (9). Here we report a similar increase in APP-751/APP-695 mRNA ratio in AD hippocampus. However, the cerebellum had a 6-fold lower ratio, which does not change in AD. The APP-695 mRNA species is greatly decreased (3-4-fold) in mRNA from AD cortex and hippocampus, whereas the APP-751 mRNA species is essentially unchanged. Considering hippocampal neuron loss and cortical neuron loss and atrophy, the data presented may be compatible with increased APP-751 mRNA and decreased APP-695 mRNA prevalence in remaining neurons in AD. A possible relationship between a high APP-751/APP-695 ratio and amyloid pathology is discussed.

Publication types

  • Corrected and Republished Article
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Alzheimer Disease / metabolism*
  • Amyloid / genetics*
  • Amyloid beta-Protein Precursor
  • Cerebellum / metabolism*
  • Cerebral Cortex / metabolism*
  • Densitometry
  • Female
  • Hippocampus / metabolism*
  • Humans
  • Male
  • Middle Aged
  • Nucleic Acid Hybridization
  • Protein Precursors / genetics*
  • RNA / isolation & purification
  • RNA Probes
  • Transcription, Genetic

Substances

  • Amyloid
  • Amyloid beta-Protein Precursor
  • Protein Precursors
  • RNA Probes
  • RNA