Monocytes and CD4+ T cells contribution to the under-expression of NR4A2 and TNFAIP3 genes in patients with multiple sclerosis

N D Navone; S Perga; S Martire; P Berchialla; S Malucchi; A Bertolotto

doi:10.1016/j.jneuroim.2014.04.017

Monocytes and CD4+ T cells contribution to the under-expression of NR4A2 and TNFAIP3 genes in patients with multiple sclerosis

J Neuroimmunol. 2014 Jul 15;272(1-2):99-102. doi: 10.1016/j.jneuroim.2014.04.017. Epub 2014 May 10.

Authors

N D Navone¹, S Perga², S Martire², P Berchialla³, S Malucchi², A Bertolotto⁴

Affiliations

¹ Neurology Unit 2 - CRESM (Regional Referring Center of Multiple Sclerosis), Neuroscience Institute Cavalieri Ottolenghi (NICO) University of Turin, AOU San Luigi Gonzaga, 10043 Orbassano, Turin, Italy. Electronic address: nicolednavone@gmail.com.
² Neurology Unit 2 - CRESM (Regional Referring Center of Multiple Sclerosis), Neuroscience Institute Cavalieri Ottolenghi (NICO) University of Turin, AOU San Luigi Gonzaga, 10043 Orbassano, Turin, Italy.
³ Department of Clinical and Biological Sciences, University of Turin, Torino, Italy.
⁴ Neurology Unit 2 - CRESM (Regional Referring Center of Multiple Sclerosis), Neuroscience Institute Cavalieri Ottolenghi (NICO) University of Turin, AOU San Luigi Gonzaga, 10043 Orbassano, Turin, Italy. Electronic address: antonio.bertolotto@gmail.com.

PMID: 24852325
DOI: 10.1016/j.jneuroim.2014.04.017

Abstract

We recently found a gene signature for multiple sclerosis (MS) that reverted to normal during pregnancy in MS patients and included NR4A2 and TNFAIP3, key molecules in anti-inflammatory processes. Here we focus on the expression levels of these two genes in monocytes and CD4+ T cells from healthy controls and treatment-naïve RRMS patients. Our findings show that monocytes play a key role in the dysregulated anti-inflammatory response, being the expression of both genes down-regulated in these cells in RRMS patients with respect to healthy individuals. CD4+ T cells seem to have only a marginal part, because we can observe only a slight down-regulation in NR4A2.

Keywords: CD4+ T cells; Monocytes; Multiple sclerosis; NR4A2; TNFAIP3.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
CD4-Positive T-Lymphocytes / metabolism*
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Disability Evaluation
Female
Gene Expression Regulation / physiology*
Humans
Intracellular Signaling Peptides and Proteins / genetics
Intracellular Signaling Peptides and Proteins / metabolism*
Male
Middle Aged
Monocytes / metabolism*
Multiple Sclerosis / pathology*
Nuclear Proteins / genetics
Nuclear Proteins / metabolism*
Nuclear Receptor Subfamily 4, Group A, Member 2 / genetics
Nuclear Receptor Subfamily 4, Group A, Member 2 / metabolism*
RNA, Messenger / metabolism
Statistics, Nonparametric
Tumor Necrosis Factor alpha-Induced Protein 3
Young Adult

Substances

DNA-Binding Proteins
Intracellular Signaling Peptides and Proteins
NR4A2 protein, human
Nuclear Proteins
Nuclear Receptor Subfamily 4, Group A, Member 2
RNA, Messenger
TNFAIP3 protein, human
Tumor Necrosis Factor alpha-Induced Protein 3