BRMS1 suppresses glioma progression by regulating invasion, migration and adhesion of glioma cells

Pengjin Mei; Jin Bai; Meilin Shi; Qinghua Liu; Zhonglin Li; Yuechao Fan; Junnian Zheng

doi:10.1371/journal.pone.0098544

BRMS1 suppresses glioma progression by regulating invasion, migration and adhesion of glioma cells

PLoS One. 2014 May 30;9(5):e98544. doi: 10.1371/journal.pone.0098544. eCollection 2014.

Authors

Pengjin Mei¹, Jin Bai², Meilin Shi², Qinghua Liu², Zhonglin Li³, Yuechao Fan³, Junnian Zheng⁴

Affiliations

¹ Department of Neurosurgery, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, China; Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou, Jiangsu, China.
² Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou, Jiangsu, China.
³ Department of Neurosurgery, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, China.
⁴ Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou, Jiangsu, China; Department of Medical Oncology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, China.

Abstract

Breast cancer metastasis suppressor 1 (BRMS1) is a metastasis suppressor gene in several solid tumors. However, the expression and function of BRMS1 in glioma have not been reported. In this study, we investigated whether BRMS1 play a role in glioma pathogenesis. Using the tissue microarray technology, we found that BRMS1 expression is significantly decreased in glioma compared with tumor adjacent normal brain tissue (P<0.01, χ(2) test) and reduced BRMS1 staining is associated with WHO stages (P<0.05, χ(2) test). We also found that BRMS1 was significantly downregulated in glioma cell lines compared to normal human astrocytes (P<0.01, χ(2) test). Furthermore, we demonstrated that BRMS1 overexpression inhibited glioma cell invasion by suppressing uPA, NF-κB, MMP-2 expression and MMP-2 enzyme activity. Moreover, our data showed that overexpression of BRMS1 inhibited glioma cell migration and adhesion capacity compared with the control group through the Src-FAK pathway. Taken together, this study suggested that BRMS1 has a role in glioma development and progression by regulating invasion, migration and adhesion activities of cancer cells.

Publication types

Research Support, Non-U.S. Gov't
Retracted Publication

MeSH terms

Astrocytes / metabolism
Astrocytes / pathology
Cell Adhesion / genetics*
Cell Line, Tumor
Cell Movement / genetics*
Disease Progression
Down-Regulation / genetics
Female
Gene Expression Regulation, Neoplastic / genetics
Genes, Tumor Suppressor / physiology
Glioma / genetics*
Glioma / pathology
Humans
Male
Matrix Metalloproteinase 2 / genetics
Middle Aged
NF-kappa B / genetics
Neoplasm Invasiveness / genetics*
Neoplasm Proteins / genetics*
Repressor Proteins
Urokinase-Type Plasminogen Activator / genetics

Substances

BRMS1 protein, human
NF-kappa B
Neoplasm Proteins
Repressor Proteins
Urokinase-Type Plasminogen Activator
Matrix Metalloproteinase 2

Grants and funding

This project is supported by grants from the National Natural Science Foundation of China (No. 81372460), the Science and Technology Department of Jiangsu province (No. BK20131120), the Health Department Foundation of Jiangsu province (No. H201019) and the Key University Science Research Project of Jiangsu Province (Nos. 11KJA320002, 12KJA320001). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.