The oncoprotein HBXIP up-regulates SCG3 through modulating E2F1 and miR-509-3p in hepatoma cells

Yue Wang; Ming Cui; Xiaoli Cai; Baodi Sun; Fabao Liu; Xiaodong Zhang; Lihong Ye

doi:10.1016/j.canlet.2014.05.007

The oncoprotein HBXIP up-regulates SCG3 through modulating E2F1 and miR-509-3p in hepatoma cells

Cancer Lett. 2014 Oct 1;352(2):169-78. doi: 10.1016/j.canlet.2014.05.007. Epub 2014 May 29.

Authors

Yue Wang¹, Ming Cui², Xiaoli Cai¹, Baodi Sun², Fabao Liu¹, Xiaodong Zhang², Lihong Ye³

Affiliations

¹ State Key Laboratory of Medicinal Chemical Biology, Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin 300071, PR China.
² State Key Laboratory of Medicinal Chemical Biology, Department of Cancer Research, College of Life Sciences, Nankai University, Tianjin 300071, PR China.
³ State Key Laboratory of Medicinal Chemical Biology, Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin 300071, PR China. Electronic address: yelihong@nankai.edu.cn.

PMID: 24882622
DOI: 10.1016/j.canlet.2014.05.007

Abstract

Hepatitis B X-interacting protein (HBXIP) is an important oncoprotein in hepatocarcinogenesis. Here, we found that the expression levels of HBXIP were positively associated with those of Secretogranin III (SCG3) in clinical hepatocellular carcinoma tissues. We identified that HBXIP up-regulated the expression of SCG3 through modulating both E2F transcription factor 1 (E2F1) and miR-509-3p. HBXIP suppressed miR-509-3p through activating NF-κB. In function, we showed that SCG3 increased the proliferation of hepatoma cells and HBXIP enhanced the proliferation of the cells via SCG3 in vitro and in vivo. Thus, we conclude that HBXIP facilitates the proliferation of hepatoma cells through up-regulating SCG3.

Keywords: E2F1; HBXIP; Hepatocellular carcinoma; SCG3; miR-509-3p.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptor Proteins, Signal Transducing / genetics
Adaptor Proteins, Signal Transducing / metabolism*
Animals
Binding Sites
Carcinoma, Hepatocellular / genetics
Carcinoma, Hepatocellular / metabolism*
Carcinoma, Hepatocellular / pathology
Cell Proliferation
Chromogranins / genetics
Chromogranins / metabolism*
E2F1 Transcription Factor / genetics
E2F1 Transcription Factor / metabolism*
Gene Expression Regulation, Neoplastic
Hep G2 Cells
Humans
Liver Neoplasms / genetics
Liver Neoplasms / metabolism*
Liver Neoplasms / pathology
Mice
Mice, Inbred BALB C
Mice, Nude
MicroRNAs / genetics
MicroRNAs / metabolism*
NF-kappa B / metabolism
Promoter Regions, Genetic
RNA Interference
RNA, Messenger / metabolism
Signal Transduction
Time Factors
Transfection
Tumor Burden
Up-Regulation

Substances

Adaptor Proteins, Signal Transducing
Chromogranins
E2F1 Transcription Factor
E2F1 protein, human
LAMTOR5 protein, human
MIRN509 microRNA, human
MicroRNAs
NF-kappa B
RNA, Messenger
SCG3 protein, human