Abstract
Carnitine/acylcarnitine translocase and carnitine palmitoyltransferase 2 are members of the carnitine system, which are responsible of the regulation of the mitochondrial CoA/acyl-CoA ratio and of supplying substrates for the ß-oxidation to mitochondria. This study, using cross-Linking reagent, Blue native electrophoresis and immunoprecipitation followed by detection with immunoblotting, shows conclusive evidence about the interaction between carnitine palmitoyltransferase 2 and carnitine/acylcarnitine translocase supporting the channeling of acylcarnitines and carnitine at level of the inner mitochondrial membrane.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Western
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Carnitine / analogs & derivatives
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Carnitine / metabolism
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Carnitine Acyltransferases / metabolism*
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Carnitine O-Palmitoyltransferase / chemistry
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Carnitine O-Palmitoyltransferase / metabolism*
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Cross-Linking Reagents / chemistry
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Formaldehyde / chemistry
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Immunoprecipitation
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Membrane Transport Proteins / chemistry
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Membrane Transport Proteins / metabolism*
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Mitochondria, Liver / enzymology*
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Mitochondrial Membranes / enzymology*
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Models, Molecular
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Native Polyacrylamide Gel Electrophoresis
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Protein Binding
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Protein Conformation
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Rats
Substances
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Cross-Linking Reagents
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Membrane Transport Proteins
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acylcarnitine
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Formaldehyde
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Carnitine Acyltransferases
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Slc25a20 protein, rat
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Carnitine O-Palmitoyltransferase
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Carnitine