Exercise-induced regulation of matrix metalloproteinases in the skeletal muscle of subjects with type 2 diabetes

Celena Scheede-Bergdahl; Andreas Bergdahl; Peter Schjerling; Klaus Qvortrup; Satu O Koskinen; Flemming Dela

doi:10.1177/1479164114535943

Exercise-induced regulation of matrix metalloproteinases in the skeletal muscle of subjects with type 2 diabetes

Diab Vasc Dis Res. 2014 Sep;11(5):324-34. doi: 10.1177/1479164114535943. Epub 2014 Jun 4.

Authors

Celena Scheede-Bergdahl¹, Andreas Bergdahl², Peter Schjerling³, Klaus Qvortrup⁴, Satu O Koskinen³, Flemming Dela⁵

Affiliations

¹ Xlab, Center for Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark Department of Kinesiology and Physical Education, McGill University, Montreal, QC, Canada celena.scheede@mcgill.ca.
² Department of Exercise Science, Concordia University, Montreal, QC, Canada.
³ Institute of Sports Medicine, Department of Orthopedic Surgery M, Bispebjerg Hospital and Center for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
⁴ Department of Biomedical Sciences/CFIM, University of Copenhagen, Copenhagen, Denmark.
⁵ Xlab, Center for Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark.

PMID: 24903024
DOI: 10.1177/1479164114535943

Abstract

Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMP) play a critical role during vascular remodelling, in both health and disease. Impaired MMP regulation is associated with many diabetes-related complications. This study examined whether exercise-induced regulation of MMPs is maintained in the skeletal muscle of patients with uncomplicated type 2 diabetes (T2DM). Subjects [12 T2DM, 9 healthy control subjects (CON)] underwent 8 weeks of physical training. Messenger RNA (mRNA) was measured at baseline, during and after 8 weeks of training. Protein was measured pre- and post-training. At baseline, there were no effects of diabetes on MMP or TIMP mRNA or protein. mRNA and protein response to training was similar in both groups, except active MMP-2 protein was elevated post training in T2DM only. Our results indicate that exercise-induced stimulation of MMPs is preserved in skeletal muscle of patients with T2DM. This early stage of diabetes may provide an opportunity for intervention and prevention of complications.

Keywords: Matrix metalloproteinase; capillary basement membrane; exercise; extracellular matrix; type 2 diabetes.

Publication types

Controlled Clinical Trial
Research Support, Non-U.S. Gov't

MeSH terms

Denmark
Diabetes Mellitus, Type 2 / enzymology
Diabetes Mellitus, Type 2 / genetics
Diabetes Mellitus, Type 2 / physiopathology
Diabetes Mellitus, Type 2 / therapy*
Enzyme Precursors / genetics
Enzyme Precursors / metabolism
Exercise Therapy*
Gelatinases / genetics
Gelatinases / metabolism
Gene Expression Regulation, Enzymologic
Humans
Male
Matrix Metalloproteinase 14 / genetics
Matrix Metalloproteinase 14 / metabolism
Matrix Metalloproteinase 2 / genetics
Matrix Metalloproteinase 2 / metabolism
Matrix Metalloproteinases / genetics
Matrix Metalloproteinases / metabolism*
Middle Aged
Muscle, Skeletal / enzymology*
Muscle, Skeletal / physiopathology
Muscle, Skeletal / ultrastructure
RNA, Messenger / metabolism
Time Factors
Tissue Inhibitor of Metalloproteinases / genetics
Tissue Inhibitor of Metalloproteinases / metabolism
Treatment Outcome

Substances

Enzyme Precursors
RNA, Messenger
Tissue Inhibitor of Metalloproteinases
Gelatinases
Matrix Metalloproteinases
progelatinase
MMP2 protein, human
Matrix Metalloproteinase 2
MMP14 protein, human
Matrix Metalloproteinase 14