Abstract
The role of Fused Toes Homolog (FTS) in epidermal growth factor (EGF) induced epithelial-mesenchymal transition (EMT) in cervical cancer cells was studied. EGF treatment induced the change of EMT markers and increased cell migration. EGF treatment also increased phosphorylated EGFR and ERK and nuclear level of ATF-2. The binding of ATF-2 to the promoter region of FTS was evidenced after EGF treatment. Pretreatment with PD98059 and gefitinib prevented EGF-induced FTS expression. FTS silencing reduced EMT and cell migration by EGF treatment. These results demonstrate a novel function for FTS in EGF-mediated EMT process.
Keywords:
Cancer cell migration; EGF; EMT; FTS; MMP.
Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / biosynthesis*
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Adaptor Proteins, Signal Transducing / genetics
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Adaptor Proteins, Signal Transducing / metabolism
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Apoptosis Regulatory Proteins / biosynthesis*
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Apoptosis Regulatory Proteins / genetics
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Apoptosis Regulatory Proteins / metabolism
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Cell Culture Techniques
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Cell Movement / drug effects
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Cell Movement / physiology
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Epidermal Growth Factor / pharmacology*
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Epithelial-Mesenchymal Transition / drug effects
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Epithelial-Mesenchymal Transition / physiology
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ErbB Receptors / metabolism
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Female
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Humans
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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RNA, Small Interfering / administration & dosage
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RNA, Small Interfering / genetics
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Transfection
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Uterine Cervical Neoplasms / genetics
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Uterine Cervical Neoplasms / metabolism*
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Uterine Cervical Neoplasms / pathology*
Substances
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AKTIP protein, human
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Adaptor Proteins, Signal Transducing
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Apoptosis Regulatory Proteins
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RNA, Messenger
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RNA, Small Interfering
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Epidermal Growth Factor
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EGFR protein, human
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ErbB Receptors