Abstract
Expression of the pS2 gene which is transcriptionally controlled by oestrogens in the breast cancer cell line MCF-7 is oestrogen independent in stomach mucosa. We show here that the level of MCF-7 cell pS2 mRNA can also be increased by the tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). We further demonstrate, using transient transfection assays, that the -428 to -332 5' flanking sequence of the pS2 gene contains DNA enhancer elements responsive to oestrogens, TPA, EGF, the c-Ha-ras oncoprotein and the c-jun protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Breast Neoplasms / genetics
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Breast Neoplasms / metabolism
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DNA, Neoplasm / genetics
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DNA-Binding Proteins / pharmacology
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Enhancer Elements, Genetic / drug effects*
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Epidermal Growth Factor / pharmacology
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Estrogens / pharmacology*
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Female
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Gene Expression Regulation / drug effects
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Humans
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Molecular Sequence Data
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Neoplasm Proteins / genetics*
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Proteins*
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Proto-Oncogene Proteins / pharmacology
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Proto-Oncogene Proteins c-jun
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Proto-Oncogene Proteins p21(ras)
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Neoplasm / genetics
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RNA, Neoplasm / metabolism
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Tetradecanoylphorbol Acetate / pharmacology
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Transcription Factors / pharmacology
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Trefoil Factor-1
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Tumor Cells, Cultured / drug effects
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Tumor Cells, Cultured / metabolism
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Tumor Suppressor Proteins
Substances
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DNA, Neoplasm
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DNA-Binding Proteins
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Estrogens
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Neoplasm Proteins
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Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-jun
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RNA, Messenger
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RNA, Neoplasm
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TFF1 protein, human
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Transcription Factors
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Trefoil Factor-1
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Tumor Suppressor Proteins
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Epidermal Growth Factor
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HRAS protein, human
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Proto-Oncogene Proteins p21(ras)
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Tetradecanoylphorbol Acetate