MicroRNA-370 suppresses proliferation and promotes endometrioid ovarian cancer chemosensitivity to cDDP by negatively regulating ENG

Cancer Lett. 2014 Oct 28;353(2):201-10. doi: 10.1016/j.canlet.2014.07.026. Epub 2014 Jul 22.

Abstract

MicroRNAs (miRNAs) are a class of non-coding RNAs that post-transcriptionally inhibit gene expression. In this study, we discovered that microRNA-370 (miR-370) was down-regulated in endometrioid ovarian cancer cells. In IGROV1 and TOV112D endometrioid ovarian cancer cells, miR-370 suppressed cellular viability and colony formation. miR-370 also enhanced endometrioid ovarian cancer cell chemosensitivity to cDDP. Endoglin (ENG) was directly and negatively regulated by miR-370. In addition, hypermethylation was a potential mechanism of miR-370 epigenetic silencing. We conclude that miR-370 acts as a tumor suppressor in endometrioid ovarian cancer via ENG regulation.

Keywords: Endoglin; Endometrioid ovarian cancer; MicroRNA; Target gene; miR-370.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD / genetics*
  • Antigens, CD / metabolism
  • Antineoplastic Agents / pharmacology*
  • Base Sequence
  • Carcinoma, Endometrioid / drug therapy
  • Carcinoma, Endometrioid / genetics*
  • Carcinoma, Endometrioid / pathology
  • Cell Line, Tumor
  • Cell Proliferation
  • Cisplatin / pharmacology*
  • Down-Regulation
  • Drug Resistance, Neoplasm
  • Endoglin
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Mice
  • Mice, Nude
  • MicroRNAs / physiology*
  • Ovarian Neoplasms / drug therapy
  • Ovarian Neoplasms / genetics*
  • Ovarian Neoplasms / pathology
  • RNA Interference
  • Receptors, Cell Surface / genetics*
  • Receptors, Cell Surface / metabolism
  • Tumor Burden
  • Xenograft Model Antitumor Assays

Substances

  • Antigens, CD
  • Antineoplastic Agents
  • ENG protein, human
  • Endoglin
  • MIRN370 microRNA, human
  • MicroRNAs
  • Receptors, Cell Surface
  • Cisplatin