Abstract
MicroRNAs (miRNAs) are a class of non-coding RNAs that post-transcriptionally inhibit gene expression. In this study, we discovered that microRNA-370 (miR-370) was down-regulated in endometrioid ovarian cancer cells. In IGROV1 and TOV112D endometrioid ovarian cancer cells, miR-370 suppressed cellular viability and colony formation. miR-370 also enhanced endometrioid ovarian cancer cell chemosensitivity to cDDP. Endoglin (ENG) was directly and negatively regulated by miR-370. In addition, hypermethylation was a potential mechanism of miR-370 epigenetic silencing. We conclude that miR-370 acts as a tumor suppressor in endometrioid ovarian cancer via ENG regulation.
Keywords:
Endoglin; Endometrioid ovarian cancer; MicroRNA; Target gene; miR-370.
Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, CD / genetics*
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Antigens, CD / metabolism
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Antineoplastic Agents / pharmacology*
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Base Sequence
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Carcinoma, Endometrioid / drug therapy
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Carcinoma, Endometrioid / genetics*
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Carcinoma, Endometrioid / pathology
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Cell Line, Tumor
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Cell Proliferation
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Cisplatin / pharmacology*
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Down-Regulation
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Drug Resistance, Neoplasm
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Endoglin
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Female
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Gene Expression Regulation, Neoplastic
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Humans
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Mice
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Mice, Nude
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MicroRNAs / physiology*
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Ovarian Neoplasms / drug therapy
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Ovarian Neoplasms / genetics*
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Ovarian Neoplasms / pathology
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RNA Interference
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Receptors, Cell Surface / genetics*
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Receptors, Cell Surface / metabolism
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Tumor Burden
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Xenograft Model Antitumor Assays
Substances
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Antigens, CD
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Antineoplastic Agents
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ENG protein, human
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Endoglin
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MIRN370 microRNA, human
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MicroRNAs
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Receptors, Cell Surface
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Cisplatin