The actin-bundling protein TRIOBP-4 and -5 promotes the motility of pancreatic cancer cells

Cancer Lett. 2015 Jan 28;356(2 Pt B):367-73. doi: 10.1016/j.canlet.2014.08.005. Epub 2014 Aug 15.

Abstract

TRIOBP isoforms 4 and 5 (TRIOBP-4/-5) are an actin-bundling protein associated with hearing loss. Here, we showed that TRIOBP-4/-5 was up-regulated in human pancreatic carcinoma cells. Knockdown of TRIOBP-4/-5 led to a loss of filopodia and a decrease in cell motility. Confocal microscopy showed that re-expression of GFP-TRIOBP-4 or -5 restored the filopodial formation in TRIOBP-4/-5-deficient PANC-1 cells. Finally, TRIOBP-4/-5 was shown to be overexpressed in human pancreatic cancer tissues. These results demonstrate a novel role of TRIOBP-4/-5 that promotes the motility of pancreatic cancer cells via regulating actin cytoskeleton reorganization in the filopodia of the cells.

Keywords: Actin cytoskeleton reorganization; Filopodia; Migration; Pancreatic cancer cells; TRIOBP-4/-5.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actin Cytoskeleton / metabolism*
  • Blotting, Western
  • Carcinoma, Pancreatic Ductal / metabolism
  • Carcinoma, Pancreatic Ductal / pathology*
  • Cell Movement*
  • Cell Proliferation
  • Humans
  • Microfilament Proteins / antagonists & inhibitors
  • Microfilament Proteins / genetics
  • Microfilament Proteins / metabolism*
  • Microscopy, Confocal
  • Pancreas / metabolism
  • Pancreatic Neoplasms / metabolism
  • Pancreatic Neoplasms / pathology*
  • Pseudopodia / metabolism
  • Pseudopodia / pathology*
  • RNA, Small Interfering / genetics
  • Tumor Cells, Cultured
  • Up-Regulation
  • Wound Healing

Substances

  • Microfilament Proteins
  • RNA, Small Interfering
  • TRIOBP protein, human