Association of CVD candidate gene polymorphisms with ischemic stroke and cerebral hemorrhage in Chinese individuals

Wenjing Ou; Xin Liu; Yue Shen; Jiana Li; Lingbin He; Yuan Yuan; Xuerui Tan; Lisheng Liu; Jingbo Zhao; Xingyu Wang

doi:10.1371/journal.pone.0105516

Association of CVD candidate gene polymorphisms with ischemic stroke and cerebral hemorrhage in Chinese individuals

PLoS One. 2014 Aug 21;9(8):e105516. doi: 10.1371/journal.pone.0105516. eCollection 2014.

Authors

Wenjing Ou¹, Xin Liu², Yue Shen², Jiana Li³, Lingbin He⁴, Yuan Yuan⁴, Xuerui Tan⁴, Lisheng Liu², Jingbo Zhao³, Xingyu Wang⁵

Affiliations

¹ Department of Epidemiology, Public Health School, Harbin Medical University, Heilongjiang, China; Department of Epidemiology, Public Health School, Harbin Medical University, Heilongjiang, China.
² The Laboratory of Human Genetics, Beijing Hypertension League Institute, Beijing, China.
³ Department of Epidemiology, Public Health School, Harbin Medical University, Heilongjiang, China.
⁴ First Affiliated Hospital, Medical College of Shantou University, Shantou, Guangdong, China.
⁵ First Affiliated Hospital, Medical College of Shantou University, Shantou, Guangdong, China; The Laboratory of Human Genetics, Beijing Hypertension League Institute, Beijing, China.

Abstract

Background: Contribution of cardiovascular disease related genetic risk factors for stroke are not clearly defined. We performed a genetic association study to assess the association of 56 previously characterized gene variants in 34 candidate genes from cardiovascular disease related biological pathways with ischemic stroke and cerebral hemorrhage in a Chinese population.

Methods: There were 1280 stroke patients (1101 with ischemic stroke and 179 with cerebral hemorrhage) and 1380 controls in the study. The genotypes for 56 polymorphisms of 34 candidate genes were determined by the immobilized probe approach and the associations of gene polymorphisms with ischemic stroke and cerebral hemorrhage were performed by logistic regression under an allelic model.

Results: After adjusting for age, sex, BMI and hypertension status by logistic regression analysis, we found that NPPA rs5063 was significantly associated with both ischemic stroke (odds ratio [OR] 0.69; 95% confidence interval [CI], 0.52 to 0.90; P = 0.006) and cerebral hemorrhage(OR = 0.39; 95%CI, 0.19 to 0.78; P = 0.007). In addition, MTHFR rs1801133 also was associated with cerebral hemorrhage (OR = 1.48; 95%CI, 1.16 to 1.89; P = 0.001) but not with ischemic stroke (OR = 1.08; 95%CI, 0.96 to 1.22; P = 0.210). After false discovery rate (FDR) correction, the association of NPPA rs5063 and MTHFR rs1801133 with cerebral hemorrhage remained significant.

Conclusions: The NPPA rs5063 is associated with reduced risk for cerebral hemorrhage and MTHFR rs1801133 is associated with increased risk of cerebral hemorrhage in a Chinese population.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Cardiovascular Diseases / complications*
Cardiovascular Diseases / genetics*
Cardiovascular Diseases / metabolism
Case-Control Studies
Cerebral Hemorrhage / epidemiology*
Cerebral Hemorrhage / etiology*
China / epidemiology
Female
Genetic Association Studies
Humans
Male
Middle Aged
Polymorphism, Genetic*
Polymorphism, Single Nucleotide
Risk Factors
Stroke / epidemiology*
Stroke / etiology*

Associated data

figshare/10.6084/M9.FIGSHARE.1111655

Grants and funding

This study was supported by the Beijing Hypertension League Institute and National Infrastructure Program of Chinese Genetic Resources (2005DKA21300). F. Hoffmann-La Roche partly funded this study through an unrestricted educational grant. There are no patents, products in development or marketed products to declare. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.