Abstract
Accumulating data have shown that microRNAs are involved in the pathogenesis of cancer. miR-202 has been confirmed to be downregulated in several types of human cancer. However, the expression and biological role of miR-202 in osteosarcoma (OS) carcinogenesis and progression remain unclear. In this study, we demonstrated that miR-202 expression is significantly decreased in human OS cell lines and specimens. Restoration of miR-202 expression could inhibit OS cell proliferation, induce cell apoptosis, and suppress tumor growth in nude mice models. We subsequently identified the transcription factor Gli2 as a direct target of miR-202. Overexpression of Gli2 blocked the inhibitory function of miR-202. Taken together, our results indicate that miR-202 acts as a novel tumor suppressor to regulate OS cell proliferation and apoptosis through downregulating Gli2 expression.
MeSH terms
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Animals
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Apoptosis*
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Bone Neoplasms / genetics
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Bone Neoplasms / metabolism*
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Bone Neoplasms / pathology
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Cell Line, Tumor
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Cell Proliferation*
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Female
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Gene Expression Regulation, Neoplastic / genetics
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Heterografts
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Humans
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Kruppel-Like Transcription Factors / biosynthesis*
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Kruppel-Like Transcription Factors / genetics
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Male
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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MicroRNAs / biosynthesis*
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MicroRNAs / genetics
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Neoplasm Proteins / biosynthesis*
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Neoplasm Proteins / genetics
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Neoplasm Transplantation
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Nuclear Proteins / biosynthesis*
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Nuclear Proteins / genetics
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Osteosarcoma / genetics
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Osteosarcoma / metabolism*
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Osteosarcoma / pathology
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RNA, Neoplasm / biosynthesis*
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RNA, Neoplasm / genetics
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Zinc Finger Protein Gli2
Substances
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GLI2 protein, human
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Kruppel-Like Transcription Factors
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MIRN202 microRNA, human
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MicroRNAs
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Neoplasm Proteins
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Nuclear Proteins
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RNA, Neoplasm
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Zinc Finger Protein Gli2