Secretory pancreatic stone protein messenger RNA. Nucleotide sequence and expression in chronic calcifying pancreatitis

J Clin Invest. 1989 Jul;84(1):100-6. doi: 10.1172/JCI114128.

Abstract

The pancreatic stone protein and its secretory form (PSP-S) are inhibitors of CaCO3 crystal growth, possibly involved in the stabilization of pancreatic juice. We have established the structure of PSP-S mRNA and monitored its expression in chronic calcifying pancreatitis (CCP). A cDNA encoding pre-PSP-S has been cloned from a human pancreatic cDNA library. Its nucleotide sequence revealed that it comprised all but the 5' end of PSP-S mRNA, which was obtained by sequencing the first exon of the PSP-S gene. The complete mRNA sequence is 775 nucleotides long, including 5'- and 3'- noncoding regions of 80 and 197 nucleotides, respectively, attached to a poly(A) tail of approximately 125 nucleotides. It encodes a preprotein of 166 amino acids, including a prepeptide of 22 amino acids. No overall sequence homology was found between PSP-S and other pancreatic proteins. Some homology with several serine proteases was observed in the COOH-terminal region, however. The mRNA levels of PSP-S, trypsinogen, chymotrypsinogen, and colipase in CCP and control pancreas were compared. PSP-S mRNA was three times lower in CCP than in control, whereas the others were not altered. It was concluded that PSP-S gene expression is specifically reduced in CCP patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Amino Acid Sequence
  • Bacteriophage lambda / genetics
  • Base Sequence
  • Blotting, Northern
  • Calcium-Binding Proteins / genetics*
  • Calcium-Binding Proteins / metabolism
  • Chronic Disease
  • Chymotrypsinogen / genetics
  • Colipases / genetics
  • DNA / genetics
  • Female
  • Gene Expression Regulation*
  • Humans
  • Lithostathine
  • Male
  • Molecular Sequence Data
  • Nerve Tissue Proteins*
  • Nucleic Acid Hybridization
  • Pancreatic Juice / analysis
  • Pancreatitis / genetics
  • Pancreatitis / metabolism*
  • Pancreatitis / pathology
  • RNA, Messenger / genetics*
  • RNA, Messenger / metabolism
  • Sequence Homology, Nucleic Acid
  • Trypsinogen / genetics

Substances

  • Calcium-Binding Proteins
  • Colipases
  • Lithostathine
  • Nerve Tissue Proteins
  • REG1A protein, human
  • RNA, Messenger
  • Trypsinogen
  • DNA
  • Chymotrypsinogen