Abstract
Cell division cycle 42 (CDC42), a small GTPase of the Rho-subfamily, regulates diverse cellular functions including proliferation, cytoskeletal rearrangement and even promotes malignant transformation. Here, we found that increased expression of CDC42 correlated with undifferentiated neuroblastoma as compared to a more benign phenotype. CDC42 inhibition decreased cell growth and soft agar colony formation, and increased cell death in BE(2)-C and BE(2)-M17 cell lines, but not in SK-N-AS. In addition, silencing of CDC42 decreased expression of N-myc in BE(2)-C and BE(2)-M17 cells. Our findings suggest that CDC42 may play a role in the regulation of aggressive neuroblastoma behavior.
Keywords:
AKT2; CDC42; N-myc; Neuroblastoma; Transformation; Twist.
Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Cell Line, Tumor
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Cell Proliferation / genetics
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Cell Transformation, Neoplastic / genetics*
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Cell Transformation, Neoplastic / metabolism
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Cell Transformation, Neoplastic / pathology*
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Humans
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N-Myc Proto-Oncogene Protein
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Neuroblastoma / genetics*
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Neuroblastoma / metabolism
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Neuroblastoma / pathology*
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism
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Oncogene Proteins / genetics
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Oncogene Proteins / metabolism
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Proto-Oncogene Proteins c-myc / genetics
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Proto-Oncogene Proteins c-myc / metabolism
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cdc42 GTP-Binding Protein / antagonists & inhibitors*
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cdc42 GTP-Binding Protein / genetics*
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cdc42 GTP-Binding Protein / metabolism
Substances
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MYCN protein, human
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N-Myc Proto-Oncogene Protein
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Nuclear Proteins
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Oncogene Proteins
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Proto-Oncogene Proteins c-myc
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cdc42 GTP-Binding Protein